原发性肝癌中MDR_1基因表达研究

Expression of MDR, gene in hepatocelhllar cardnoma

探讨MDR_1基因编码的P-糖蛋白(Pgp)在肝癌(HCC)及癌周正常肝组织中的表达及其临床意义。用免疫组化SABC法,检测78例肝癌及60例癌旁组织中Pgp表达,分析肝癌中Pgp表达程度和临床病理特征。(1)Pgp表达主要位于肝癌、胆管上皮细胞膜上,少量位于胞质内;Pgp表达量,在治疗前癌组织中平均为26％,而癌旁组织平均为56％以上,治疗后接近癌旁组织。(2)Pgp表达量与肝癌分化程度有关,分化程度越高,其表达量越高。(3)术后1、3、5年,Pgp高表达组生存率略高于低表达组。

To explore the expression and clinical significance of Pgp in hepatocellular carcinoma (HCC) and its surrounding liver tissues. The expression of Pgp in 78 cases of HCC and 60 surrounding normal liver tissues was detected with immunohistochemical SABC. The relationship between Pgp expression in HCC tissues and clinicopathological features of the tumors was also analyzed. The expression of Pgp mainly lied on the membrance of carcinoma and biliary duct cells and a few in plasma. Pgp positive rate in liver tissues was significantly higher than that in carcinoma tissues in untreated patients(56.4%VS26.0% ,U=2.65,P<0.01). The positive rate of Pgp in tumor tissue after treatment was over 50% . The expression of Pgp in carcinoma tissues was only related to tumor cell differentiation ( x2= 5.87, P < 0.05 ) . Liver is an intrinsic drug - resistance organ, and durg - resistance of HCC was enhanced after treatment. A reasonable chemotherapy protocol should be selected.