摘要
提出了高效液相色谱法测定玫瑰花中9种黄酮含量的方法。玫瑰花样品经甲醇-水(8+2)混合液高速匀浆提取,分取提取液5.0mL,经MCI-GEL反相树脂固相萃取小柱净化,取净化液2.00mL供色谱分析。用Waters Exterra色谱柱为分离柱,用甲醇和乙酸(0.5+99.5)溶液以不同比例混合的混合液为流动相进行梯度洗脱,在检测波长358nm处进行测定。9种黄酮在一定的质量浓度范围内与其峰面积呈线性关系。方法的检出限(3S/N)在35~45μg·L-1之间。加标回收率在96.3%~106%之间,测定值的相对标准偏差(n=7)在1.8%~2.8%之间。
A method of HPLC for the determination of 9flavones in rose was proposed.The sample was extracted with mixture of methanol-H2O(8+2)by speedy and homogeneous pulping.An aliquot of 5.0mL of the extract was purified by passing through MCI-GEL RP-resin SPE column.Waters Exterra chromatographic column was used as stationary phase with mixtures of methanol and acetic acid(0.5+99.5)solution with different mixing ratios were used as mobile phase in the gradient elution.UV-detection at 358nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 9flavones were kept in definite ranges.Detection limits(3S/N)found were ranged from 35to 45μg·L-1.Values of recovery found by standard addition method were in the range of 96.3%-106%,and values of RSD′s(n=7)in the range of 1.8%-2.8%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2014年第3期353-356,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
