分光光度法间接快速测定过氧化氢——基于纳米α-FeOOH/GO的类似过氧化物酶的催化作用

Indirect and Rapid Spectrophotometric Determination of Hydrogen Peroxide——Based on the Catalytic Action of Mimetic Peroxidase of Nanoparticles of α-FeOOH/GO

试验表明用超声原位沉淀法制备的α-FeOOH/GO纳米颗粒具有类似于辣根过氧化物酶的活性,且能催化活化过氧化氢而使底物N,N-二乙基-p-苯二胺氧化生成在550nm处有吸收峰的有色化合物。在反应条件下,过氧化氢的浓度在2.0×10-7~2.0×10-4 mol·L-1范围内与吸光度呈线性关系,检出限(3S/N)为1.0×10-7 mol·L-1。据此提出了用分光光度法间接、快速测定过氧化氢含量的方法。应用此方法测定了雨水和蜂蜜样品中过氧化氢的含量,并用标准加入法进行回收试验,测得回收率在92.3%~108%之间,测定值的相对标准偏差(n=5)均小于5%。

It was found that nanoparticles ofα-FeOOH/GO,prepared by ultrasonic in situ precipitation,behaved to have the activity mimetic to HRP and to catalytically activate H2O2,by which the base compound,N,Ndiethyl-p-phenylenediamine was oxidized to give a colored compound with absorption maximum at 550nm.Under the optimized reaction conditions.Linear relationship between values of absorbance and concentration of H2O2 was obtained in the range of 2.0×10-7-2.0×10-4 mol·L-1,with detection limit(3S/N)of 1.0×10-7 mol·L-1.Based on these facts a rapid spectrophotometric method for determination of H2O2 was proposed.The proposed method was applied to determination of H2O2in samples of rainwater and honey.Recovery was tested by standard addition method,giving results of recovery in the range of 92.3%-108%,with values of RSD′s(n=5)less than5%.