衰减全反射-傅里叶变换红外光谱法测定橙子油中柠檬烯的含量

ATR-FTIR Determination of Limonene in Orange Oil

应用衰减全反射傅里叶变换红外光谱法测定橙子油中柠檬烯的含量。柠檬烯的特征峰(1 645.1±1)cm-1为定量测量峰,测定样品在15min内完成。柠檬烯的体积分数在9.60%~76.8%范围内与测量峰峰高呈线性关系。以橙子油样品为基体进行加标回收试验,所得回收率在99.7%~101%之间,测定值的相对标准偏差(n=5)为1.1%。

FTIR with attenuated total reflection(ATR-FTIR)was applied to the determination of limonene in orange oil.The characteristic peak of limonene at(1 645.1±1)cm-1 was chosen as the quantitative peak,and the process of analytical sample was finished within 15 min.Linear relationship between values of peak height and volume fraction of limonene was kept in the range of 9.60%-76.8%.On the base of the sample of orange oil,test for recovery was made by standard addition method;values of recovery found were in the range of 99.7%-101%,with RSD(n=5)of 1.1%.