Emerson试剂分光光度法测定头孢羟氨苄

Spectrophotometric Determination of Cefadroxil with Emerson Reagent

采用Emerson试剂分光光度法测定头孢羟氨苄的含量。在pH 9.60的四硼酸钠-氢氧化钠缓冲溶液中,在铁氰化钾存在下,头孢羟氨苄可与4-氨基安替比林发生反应,生成红色的化合物,组成比为1比1,最大吸收波长为508nm。头孢羟氨苄的质量浓度在1.20~110.0mg·L-1范围内与其吸光度呈线性关系。方法的检出限(3s/k)为0.80mg·L-1。方法应用于头孢羟氨苄胶囊中头孢羟氨苄含量的测定,测定结果与药典法测定值相符,测定值的相对标准偏差(n=5)在0.20%~0.71%之间。Emerson试剂与头孢羟氨苄的反应机理被初步探讨。

Spectrophotometry was applied to the determination of cefadroxil with emerson reagent.In Na2B4O7-NaOH buffer solution of pH 9.60,a red compound was produced by the reaction of cefadroxil with 4-aminoantipyrine in the presence of K3Fe(CN)6.The stoichiometric ratio of the compound was 1∶1and the maximum absorption wavelength found was 508 nm.Linear relationship between values of absorbance and mass concentration of cefadroxil was obtained in the range of 1.20-110.0mg·L-1,with detection limit(3s/k)of0.80mg·L-1.The proposed method was applied to the analysis of drug samples of cefadroxil,giving results in consistency with the values obtained by pharmacopoeia method with RSD′s(n=5)in the range of 0.20%-0.71%.Reaction mechanism of Emerson reagent and cefadroxil was also studied preliminarily.