摘要
建立了固相萃取-高效液相色谱法测定化妆品中鞣花酸的含量。样品用甲醇-水(1+1)溶液提取,超声提取10min,以10 000r·min-1转速离心5min,移取5.00mL上清液,经MAX固相萃取柱处理,用甲酸-甲醇(5+95)溶液洗脱。MG C18柱(250mm×4.6mm,5μm)为固定相,以0.2%(体积分数)磷酸-乙腈(18+82)溶液为流动相进行洗脱,检测波长为254nm。线性范围为0.5~100mg·L-1,测定下限(10S/N)为20mg·kg-1,应用该方法对化妆品中鞣花酸的含量进行检测,加标回收率在83.5%~100%之间。
A SPE-HPLC method of determining ellagic acid in cosmetics was developed.Samples were extracted with ultrasonic for 10 min by the mixture of methanol and water(1+1),then centrifugated at 10 000r·min-1 for 5min.5.00 mL of supernatant was treated by MAX solid phase extraction,then eluted by formic acidmethanol(5+95)solution.The sample extraction was analyzed by MG C18column(250mm×4.6 mm,5μm).The mobile phase used for washing was 0.2% phosphate acid-acetonitrile(18+82),with detection wavelength at254 nm.A good linear relationship was obtained from 0.5-100mg·L-1.The limit of quantitation(10S/N)was20mg·kg-1.Recovery of the method found was in the range of 83.5%-100%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2014年第11期1366-1368,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家质检总局公益性行业科研专项(2012104013-2)
