固相萃取-高效液相色谱法同时测定环境水体中微量药品和个人护理用品

Simultaneous Determination of 10 Pharmaceuticals and Personal Care Products in Environmental Waters by SPE-HPLC

结合固相萃取与高效液相色谱分析技术,建立了一种可以同时检测环境水体中10种微量药品和个人护理用品(PPCPs)的分析方法。将水样pH调节至5,过Poly-Sery HLB固相萃取小柱,用6mL的甲醇洗脱。洗脱液在Agilent Eclipse XDB-C18色谱柱上分离,以水-乙腈为流动相进行梯度洗脱,检测波长为224nm。10种目标化合物的质量浓度在0.5~20mg·L-1范围内与其峰面积呈线性关系。方法的检出限(3S/N)为6.0~54.6μg·L-1,测定下限(10S/N)为20.1~182.1μg·L-1。对自来水和地表水进行加标试验,回收率分别在71.2%~108%和56.7%~97.6%之间。方法用于某城镇污水处理厂的原水和最终处理出水的检测。

A method of solid-phase extraction(SPE)combined with high-performance liquid chromatography(HPLC)was developed for simultaneous determination of 10 pharmaceuticals and personal care products(PPCPs)in environmental waters.The water samples with pH of 5 were purified by SPE on Poly-Sery HLB column and eluted with 6mL of methanol.The elution was separated using Agilent Eclipse XDB-C18 as stationary phase and a mixture of water and acetonitrile as mobile phase for gradient elution.UV-detection at 224 nm was recorded.The linear relationship between the peak area and the mass concentration of 10 PPCPs were in the range of 0.5-20mg·L-1,with detection limits in the range of 6.0-54.6μg·L-1 and lower limits of determination(10S/N)in the range of 20.1-182.1μg·L-1.Tests for recovery were made by adding standard samples to running water and surface water,giving values of recovery of 71.2%-108% and 56.7%-97.6%,respectively.The method was applied to assess the raw water and treated water of a sewage treatment plant.