固相萃取-高效液相色谱法测定中药制剂及保健食品中芍药苷

HPLC Determination of Peoniflorin in Traditional Chinese Medicine and Health Food with Solid Phase Extraction

应用固相萃取-高效液相色谱法测定中药制剂及保健食品中芍药苷的含量。样品经30%(体积分数)乙醇溶液提取,酸性氧化铝固相萃取柱净化。以C18色谱柱为分离柱,用乙腈和0.1%(体积分数)磷酸溶液以不同比例混合的溶液为流动相进行梯度洗脱,在检测波长230nm处进行测定。芍药苷的质量浓度在2.00~200mg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)为0.5mg·L-1。加标回收率在99.5%~101%之间,测定值的相对标准偏差(n=6)在均小于2%。

HPLC was applied to the determination of peoniflorin in traditional Chinese medicine and health food with solid phase extraction.The sample was extracted with 30%(φ)ethanol solution,and purified by passing through acidic Al2O3 solid phase extraction column.C18 chromatographic column was used as stationary phase with mixtures of acetonitrile and 0.1%(φ)H3PO4 solution with different mixing ratios as mobile phase in gradient elution.UV-detection at 230 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of peoniflorin was kept in the range of 2.00-200mg·L-1,with detection limit(3S/N)of0.5mg·L-1.Values of recovery found by standard addition method were in the range of 99.5%-101%,and values of RSD′s(n=6)less than 2%.