毛细管电泳-间接紫外法测定饮用水中草甘膦、草铵膦及其代谢产物

Determination of Glyphosate,Glufosinate and Their Metabolites by Capillary Electrophoresis Combined with Indirect UV Detection

提出了毛细管电泳-间接紫外分析方法测定饮用水中草甘膦及其代谢产物氨甲基膦酸、草铵膦及其代谢产物3-甲基磷酸亚基丙酸。样品与25mmol·L-1乙酸铵溶液混匀后,采用SAX型固相萃取小柱提取待测物。洗脱液经毛细管电泳分离,以o-磷酸基-DL-苏氨酸为内标物,紫外检测波长为214nm。草甘膦、草铵膦、氨甲基膦酸和3-甲基磷酸亚基丙酸的线性范围为0.10~20.0mg·L-1,检出限(3S/N)分别为0.22,0.30,0.18,0.03mg·L-1,加标回收率在82.1%~108%之间。

A method of capillary electrophoresis combined with indirect UV detection was developed for the determination of glyphosate, glufosinate, and their metabolites(aminomethylphosphonic acid and 3-methylphosphinicopropionic acid).The sample was mixed with ammonium acetate solution of 25mmol·L-1 and the target compounds in the mixture were extracted with SAX cartridge solid phase extraction column.The eluent was separated by capillary electrophoresis with o-phosphate-DL-threonine as internal standard.UV detection was made at the wavelength of 214 nm.The linearity ranges of glyphosate,glufosinate,aminomethylphosphonic acid and 3-methylphosphinicopropionic acid were 0.10-20.0 mg·L-1.The detection limits were 0.22,0.30,0.18,0.03mg·L-1,respectively.Recovery rates measured by standard addition method were in the range of 82.1%-108%.