摘要
采用高效液相色谱法测定地骨皮中18种氨基酸的含量。地骨皮样品用10-乙基吖啶酮-2-磺酰氯为柱前衍生试剂进行衍生化。以Akasil-C18色谱柱为固定相,用乙腈(5+95)溶液和乙腈(95+5)溶液以不同比例混合的溶液为流动相进行梯度洗脱,用荧光检测器测定。18种氨基酸的线性范围均为32fmol^100pmol,检出限(3S/N)在2.06~8.65fmol之间。加标回收率在96.4%~107%之间,测定值的相对标准偏差(n=6)在均小于4.0%。
HPLC was applied to the determination of 18 amino acids in cortex lycii.The sample of cortex lycii was derived with 10-ethyl-acridine-2-sulfonyl chloride as precolumn derivatization reagent. Akasil-C18 chromatographic column was used as stationary phase with mixtures of acetonitile(5+95)solution and acetonitile(95+5)solution with different mixing ratios as mobile phase in the gradient elution,and determined with fluorescence detector.Linearity ranges of the 18 amino acides were found in the same range of 32fmol-100 pmol with detection limits(3S/N)in the range of 2.06-8.65 fmol.Values of recovery found by standard addition method were in the range of 96.4%-107%,and values of RSD′s(n=6)less than 4.0%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2015年第10期1444-1447,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
