HPLC法测定复方黄连素注射液中甲氧苄啶和硫酸氢黄连素的含量

HPLC Determination of Trimethoprim and Berberine Sulfate in Compound Berberine Injection

采用高效液相色谱法分离复方黄连素注射液中的甲氧苄啶和硫酸氢黄连素 ,色谱柱为KromasilODS( 5 μm ,4 6mm× 2 5 0mm) ,流动相为乙腈— 0 0 0 5mol·L-1庚烷磺酸钠溶液 ( 4 0∶60 ,用冰醋酸调节pH至3 0 ) ,流速 1 0ml·min-1,检测波长 2 70nm。线性范围甲氧苄啶 7 5 1～ 15 0 12 μg·ml-1,r =0 9999;硫酸氢黄连素 2 5 1～ 5 0 14μg·ml-1,r =0 9999。回收率甲氧苄啶 10 0 3%;硫酸氢黄连素 99 9%。本方法简便 ,结果准确可靠 ,适用于该制剂 2组分的同时测定。

An external standard HPLC method has been established for the determination of trimethoprim and berberine sulfate in compound berberine injection. The analysis was carried on a Kromasil C 18 column(250mm×4.6mm,5μm) and detected at 270nm. The mobile phase is acetonitril-0.005mol·L -1 sodium heptanesulfonate (40∶60), pH was adjusted to 3.0 with acetic acid. The flow rate was 1.0ml·min -1. The linear correlation was good in the range of 7.51～150.124μg and 2.51～50.14μg for trimethoprim(r=0.9999) and berberine sulfate(r=0.9999) respectively. The average recovery was 100.3% and 99.9%, RSD was 0.5% and 0.6%(n=5) respectively. The method is simple, accurate and suitable for determination of the two constituents in compound preparation.