期刊文献+

尖吻蝮蛇(Agkistrodon acutus)毒出血毒素I出血机制的研究 被引量:3

Studies on the Hemorrhagical Mechanisim of Hemorrhagical Toxix I from Agkistrodon Acutus Venom
下载PDF
导出
摘要 用125I标记尖吻蝮蛇毒出血毒素I(AaHI),用125I-AaHI为材料研究它在家兔体内的分布和药物代谢动力学,研究结果表明,有血脑屏障存在,肝脏和肾脏含量较高,125I-AaHI代谢的产物主要通过肾排出,经计机模拟,125 I-AaHI的药物代谢动力学为一室模型,其中生物半衰期T1/2 为53.1min,K值为0.0154min-1.结合动力学研究结果表明,离子强度、温度和离子种类对125I-AaHI与肾匀浆组织结合无影响,pH低于5.8或高于8.0时结合能力下降,结合有时间饱和性,并有明显的竞争抑制现象.上述结果说明125I-AaHI在动物体内均匀分布,可能有AaHI相关的结合位点或受体存在. Hemorrhagin I(AaH I)isolated from Agkistrodon acutus venom was labeled with  125I and 125I-AaH I was given to rabbits by intravenous injectiom.The distribution of the toxin in the rabbits,the pharmacokinetic and the binding kinetics were studied.The results indicate that the blood brain barrier exists and most of 125I-AaH I metabolites is eliminated by kidney.For pharmacokinetics,the compartment-simulated curve demonstrates that the result corresponds to one compartment model.The biological half-life T is 53.1min,and K is 0.0154 min-1.The results in binding kinetics show that temperature,ionic strengh and different ions had no influences to the combination of 125I-AaH I with homogenized kidney,which is rich in blood capillary.pH had some influence under the condition of above 8.0 or below 5.8. There were time-dependent and concentration-dependent saturation properties.The unlabeled AaH I can inhibited the binding of 125I-AaH I with homogenized kidney.We concluded that the distribution of the toxin in rabbits is even except brain,and there may been some corresponding receptors or binding sites to AaH I in animal body.
出处 《科技通报》 北大核心 2003年第2期154-157,161,共5页 Bulletin of Science and Technology
关键词 出血毒素Ⅰ(AaHⅠ) 药物代谢动力学 结合动力学 Hemorrhagin I (AaH I) pharmacokinetics binding kinetics
  • 相关文献

参考文献6

  • 1Xu Xun,Wang Chun,Liu Jing,et al.Purification and Characteriza tion of Hemorrhagic Components from Agkistrodon acutus(Hundred Pace Snake)Venom[J].Toxcon,1981 ,19:623~644.
  • 2Xu Xun ,Zhu Yanhua,Wang Yuzhen,et al.Study on the chemical m odification of hemo rrhagic toxin I from five pace snake(Agkistrodon acutus)venom[J].Toxicon,1 985,23:283~288.
  • 3Liu Qingdu ,Xu Weihua,Cheng Xin.Molecular cloning and sequence ana lysis of cDNA e ncoding hemorrhagic toxin acutolysin a from agkistrodon acutus venom[J].T oxicon,1999,37:1539~1548.
  • 4何华平 王玉珍 徐询.酶标法观察尖吻蝮出血毒素I对毛细血管的作用.动物学研究,1988,9:99-101.
  • 5Kondo H,Kondon S,Ikezawa H,et al.Studies on the quantitative method in determinat ion of hemorrhagic activity of habu snake venom[J].Jap J Med Sci Biol,1960,13: 43~48.
  • 6陈刚.表观分布容积[A].陈刚.治疗药物监测[M].北京:人民军医出版社,1988.61~68.

同被引文献7

引证文献3

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部