弱精子症男性不育患者免疫相关精子抗原的筛查

Screening for the immune associated sperm antigen on the male asthenozoospermia infertility

目的观察抗精子抗体阳性血清与弱精子症患者精子抗原的结合情况,初步探讨与男性不育弱精子症患者相关的精子抗原。方法酶联免疫试验方法筛选抗精子抗体阳性血清标本,混合抗球蛋白反应试验确认抗精子抗体阳性血清。SDS-PAGE电泳分离弱精症患者和正常生育者精子蛋白,抗精子抗体阳性血清作为一抗,进行免疫印迹试验,化学发光反应显示相应精子抗原的不同反应条带,分析弱精症和正常生育者精子抗原与抗精子抗体阳性血清反应条带的差异。结果用ELISA法抗精子抗体检测试剂盒检测不明原因不孕女性血清432例,筛出抗精子抗体阳性血清44例,阳性率为10.19%。精子抗原免疫印迹试验显示,在11-17k D、35-48k D、48-63k D、63-75k D之间出现反应条带的阳性数在弱精症组和正常生育组间存在明显的统计学差异(P<0.05),而在17-25k D、25-35k D之间出现条带阳性数在两组间无明显统计学差异。结论弱精子症患者与正常正育组间11-17k D、35-48k D、48-63k D、63-75k D间精子抗原的差异可能是弱精子症发生的原因,进一步的研究有助于揭示弱精子症发生的机制。

Objective The current research focused on sperm antigens associated with male immunological infertility.Methods SDS-PAGE was performed on 103 sperm samples from patients with asthenozoospermia and 29 from fertile men.Analysis differential human sperm antigens reacting with sera from antisperm antibody positive patients by Western Blotting technique.All the results were subject to statistical analysis.Results A statistically significant difference was found in these four bands of 11-17 k D 、 35-48 k D 、 48-63 k D 、 63-75 k D between donors with normal fertility and patients with asthenozoospermia(P<0.05).No statistically significant difference between two groups in the bands of 17-25 k D、25-35 k D.Conclusion The size of sperm antigens associated with fertility may in these four bands 11-17 k D、35-48 k D、48-63 k D、63-75 k D.