长时程心脏骤停复苏后家猪心脏线粒体的影响

Effects of Mitochondria in the Heart of Pigs after Long-term Cardiac Arrest

目的:建立长时程心脏骤停复苏家猪模型,探究长时程心肺复苏后家猪线粒体及其功能的变化。方法:通过右心室交流电致颤法建立长时程10分钟室颤家猪模型,将10头家猪分为假手术组(Sham组)和复苏组(ROSC组),Sham组不经历心脏骤停和复苏直接取材,ROSC组的动物经历10分钟未处理室颤和复苏后监测12小时后取材。检测两组动物的ATP水平和线粒体超微结构。结果:复苏后与Sham对比,ROSC的12小时心脏线粒体水平明显下降。线粒体超微结构显示复苏后12小时心肌线粒体广泛肿胀,体积增大,形状不一,肿胀严重者呈圆形;线粒体嵴部分断裂,结构不清晰,基质密度降低。结论:长时程心脏骤停复苏后家猪12小时后线粒体损伤普遍存在。

Objective:To establish a long-term cardiac arrest resuscitation pig model to investigate the changes of mitochondria and their functions in long-term cardiopulmonary resuscitation.Methods:A long-term 10-minute ventricular fibrillation pig model was established by right ventricular AC fibrillation.Ten pigs were divided into sham operation group(Sham group)and resuscitation group(ROSC group).Sham group did not undergo cardiac arrest.The animals in the ROSC group were subjected to 10 minutes of untreated ventricular fibrillation and 12 hours after resuscitation monitoring.ATP levels and mitochondrial ultrastructure were measured in both groups of animals.Results:Compared with Sham after resuscitation,the 12-hour cardiac mitochondria level of ROSC decreased significantly.The mitochondrial ultrastructure showed that the myocardial mitochondria were extensively swollen,volume increased,and the shape was different 12 weeks after resuscitation.The swelling was severe and the mitochondria were partially broken,the structure was not clear,and the matrix density was decreased.Conclusion:Mitochondrial damage is common in pigs after 12 hours of long-term cardiac arrest.