铜肽PLGA纳米粒子对黑素细胞黑色素合成作用的研究

Study on the effects of melanogenesis in melanocyte induced by GHK-Cu-PLGA nanoparticles

铜肽(GHK-Cu)是甘氨酰组氨酰赖氨酸三肽与铜的络合物,具有多种生物活性.以乳酸-乙醇酸共聚物(PLGA)为载药材料制备铜肽PLGA纳米粒子,并研究其对黑素细胞酪氨酸酶活性和黑色素合成的作用.实验结果表明:铜肽对蘑菇酪氨酸酶具有激活作用;PLGA纳米粒子中铜肽的包封率为40.12%,粒径大小为100～200nm,在透射电镜下为大小均匀的圆形粒子.铜肽PLGA纳米粒子可以将铜肽成功导入黑色素细胞内,激活酪氨酸酶的活性并增加黑色素的含量.研究结果为研究铜肽PLGA纳米粒子对色素减退或色素脱失性皮肤病的治疗作用提供了初步的实验依据.

Glycyl-histidyl-lysine(GHK)-Cu has various biological activities.In this study,polylactide-co-glycolide(PLGA)has been used as carrier for encapsulating GHK-Cu to prepare GHK-CuPLGA nanoparticles and the effects of tyrosinase and melanogenesis in melanocyte were analyzed.The results showed that GHK-Cu could activate mushroom tyrosinase.The entrapment efficiency of GHK-Cu-PLGA nanoparticles was 40.12%,and particle size was 100~200 nm.Round with uniform size particles were observed under transmission electron microscope.GHK-Cu-PLGA nanoparticles were successfully delivered GHK-Cu into melanocyte,stimulated tyrosinase and increased melanogenesis.Our results provide the evidence that GHK-Cu-PLGA nanoparticles could be utilized as a treatment for hypomelanosis or depigmentation disorder.