摘要
辽宁营口某猪场发生猪消瘦、呼吸系统疾病甚至死亡的疫情。采集病料提取病变组织总DNA或RNA进行猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪细小病毒、猪伪狂犬病病毒、猪瘟病毒、猪肺炎支原体的PCR或RT-PCR检测。PCR扩增出353 bp的猪圆环病毒2型特异性条带和670 bp的猪支原体肺炎特异性条带。诊断为猪圆环病毒2型和猪肺炎支原体混合感染。
A pig farm in Yingkou broke out disease which caused swine weight loss, disease of re-spiratory system, even death. Samples of diseased pigs were collected. Total DNA and RNA of the samples were extracted. Porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2(PCV2), classic swine fever virus(CSFV), pseudorabies virus(PRV), porcine parvo-virus(PPV) and mycoplasma hyopneumoniae(Mhp), were detected by PCR or RT-PCR. A 353 bp production which correspond to porcine circovirus type 2 was amplified and a 670 bp production which corre-spond to mycoplasma hyopneumoniae was amplified. Finally, the diagnosis was a mixed infection of PCV2 with MHP.
出处
《现代畜牧兽医》
2015年第1期40-43,共4页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
猪圈环病毒2型
猪肺炎支原体
混合感染
PCR
诊断
Porcine circovirus type 2
Mycoplasma hyopneumoniae
Mixed infection
PCR
Diagnosis
