探究Eph受体A2在结直肠癌细胞化疗耐药中的作用及相关机制

To explore the role and mechanism of Eph receptor A2 in chemoresistance of colorectal cancer cells

目的探讨Eph受体A2在结直肠癌细胞化疗耐药中的作用及相关机制。方法选择中国科学院上海生命科学研究院细胞资源中心提供且本实验室自行传代冻存的人结直肠癌细胞株、结肠耐药细胞株进行实验。观察两种细胞株的半数抑制浓度、Eph受体A2表达水平,并观察5-氟尿嘧啶不同浓度下亲本细胞株中Eph受体A2蛋白表达水平。结果结肠癌耐药细胞株的半数抑制浓度优于亲本株,差异有统计学意义(P<0.05)。结肠癌亲本细胞株Eph受体A2蛋白表达水平(1.08±0.73),结肠癌耐药细胞株Eph受体A2蛋白表达水平(2.57±0.81)差异有统计意义(t=6.843,P=0.000)。结论人Rph受体A2基因可增加结直肠癌肿瘤细胞耐药性,这可能与EMT有关。值得临床重视。

Objective To investigate the role and mechanism of Eph receptor A2 in chemoresistance of colorectal cancer cells.Methods Human colorectal cancer cell lines and colon resistant cell lines provided by the Cell Resource Center of Shanghai Institute of Life Sciences,Chinese Academy of Sciences and frozen by our laboratory were selected for experiments.The half inhibition concentration and Eph receptor A2 expression level of the two cell lines were observed,and the Eph receptor A2 protein expression level in the parent cell line was observed at different concentrations of 5-fluorouracil.Results the half inhibition concentration of colon cancer resistant cells was significantly higher than that of their parents(P<0.05).The expression level of Eph receptor A2 protein in colon cancer parent cell line(1.08±0.73)was significantly different from that in colon cancer resistant cell line(2.57±0.81)(t=6.843,P=0.000).Discussion Human Rph receptor A2 gene can increase drug resistance of colorectal cancer cells,which may be related to EMT.It is worthy of clinical attention.