4种不同中药复方对慢性应激模型大鼠行为学的影响

Effects of Four Kinds of Traditional Chinese Herbal Compound Formulae on Ethology of Chronic Stress Model Rats

目的:探讨四逆散、四君子汤、柴芍六君子汤、右归丸对慢性应激大鼠行为学的影响。方法:90只Wistar雄性大鼠随机分为空白组、模型组、右归丸组、四逆散组、四君子汤组、柴芍六君子汤组6组,建立慢性应激动物模型,观察大鼠体质量及行为学指标变化。结果:与空白组相比,模型组大鼠体质量增长缓慢,糖水偏好率、旷场水平运动总路程、十字高架迷宫开臂滞留时间、水迷宫穿越平台次数、目标象限路程及停留时间均显著减少(P <0. 05),和模型组相比,各用药组体质量增长差异无统计学意义,四逆散组、四君子汤组糖水偏好率升高(P <0. 05),各用药组旷场水平运动总路程均增加(P <0. 05);四逆散、四君子汤、柴芍六君子汤组的开臂滞留时间延长(P <0. 05);柴芍六君子汤、右归丸组穿越平台次数、目标象限路程及停留时间增多(P <0. 05)。结论:四逆散、四君子汤、柴芍六君子汤、右归丸4种方药均能有效地改善模型大鼠的自主活动;其中四逆散和四君子汤可有效提高模型大鼠对奖赏的反应性;四逆散、四君子汤、柴芍六君子汤可改善模型大鼠焦虑样行为;右归丸、柴芍六君子汤具有改善模型大鼠空间学习记忆力的作用。4种中药复方中,四逆散、四君子汤、柴芍六君子汤具有较好的抗应激损伤的作用。

Objective:To investigate the effects of Sini Powder,Sijunzi Decoction,Chaishao Liujunzi Decoction and Yougui Pills on the ethology of chronic stress rats.Methods:Ninety male Wistar rats were randomly divided into 6 groups,including blank group,model group,Yougui Pills group,Sini Powder group,Sijunzi Decoction group and Chaishao Liujunzi Decoction group.Except for blank groups,the other five groups were exposed to chronic and comprehensive stress to form depression model.Changes of body weight as well as ethology were observed.Results:Compared with the blank group,the body weight of the model group increased slowly.The sucrose preference rate,the total horizontal distance of the open field,the time of the crossover elevated maze arm stay,the number of the water maze crossing the platform,the distance of the target quadrant,and the residence time were significantly reduce(P<0.05).Compared with the model group,there was no statistically significant difference in the body mass gain of each treated group while the sucrose preference rate in the Sini Powder group and the Sijunzi Deoction group increased,and the total distance of the horizontal movement of the treated groups accelerated.The time spent in the open-arm stay of the Sini Powder,Sijunzi Decoction and Chaishao Liujunzi Decoction groups was prolonged.The number of crossing platforms,the target quadrant distance and the residence time of the Chaishao Liujunzi Decoction and Yougui Pills groups increased.Conclusion:Sini Powder,Sijunzi Decoction,Chaishao Liujunzi Decoction and Yougui Pills can effectively improve the spontaneous activities of model rats.Sini Powder and Sijunzi Decoction can effectively increase the responsiveness to rewards.The anxiety-like behavior in model rats can be ameliorated by Sini Powder,Sijunzi Decoction,Chaishao Liujunzi Decotion.The spatial learning and memorization can be enhanced by Yougui Pills and Chaishao Liujunzi Decoction.Among the four traditional Chinese herbal formulae,Sini Powder,Sijunzi Decoction and Chaishao Liujunzi Decoction have better effect on stress injury.