基于网络药理学探究养心通脉方对冠心病的作用机制

Study of Yangxin Tongmai Formula on Coronary Heart Disease Based on Network Pharmacology

目的:利用网络药理学技术更全面地预测养心通脉方(Yangxin Tongmai Formula,YTF)对冠心病(coronary heart disease,CHD)潜在的作用机制。方法:利用药物靶点数据库分析平台,基于化合物ADME特性筛选养心通脉方中相关活性成分,预测化合物的作用蛋白质靶点,进一步构建靶点蛋白数据集;基于Genecards数据库获取CHD高度相关蛋白靶点,构建数据集;将养心通脉方与CHD靶点数据集进行比对与分析,并基于共同靶点构建蛋白质-蛋白质互作网络(Protein-protein interaction,PPI)网络,靶点通路网络(target-pathway network)及进行生物信息学分析。结果:经分析养心通脉方中62个化合物,99个作用蛋白质靶点以及与CHD相关的20个共同靶点(占养心通脉方化合物靶点的25%)发现,相关靶点共有55个的生物学过程及39条通路被显著富集(P <0. 05),本次预测结果与养心通脉方前期已证实的成果在功效及机制上有一定契合。结论:本研究基于网络药理学通,过构建多维调控网络初步揭示了养心通脉方对CHD的作用机制,为其的临床应用提供了理论依据。

Objective:To predict the potential theraputic mechanism of Yangxin Tongmai Formula(YTF)on coronary heart disease(CHD)using network pharmacology.Methods:Drugs and targets analysis databases were used to screen the bioactive components in YTF based on ADME characteristics and predict their corresponding targets.Meanwhile,the CHD highly related targets were obtained from Genecards database.Based on the common targets of CHD and YTF target,the protein-protein interaction(PPI)network and target-pathway network were constructed for a futher bioinformatics analysis.Results:After analyzing 62 compounds,99 protein targets and 20 common targets related to CHD(accounting for 25%of the target of YTF compound),bioinformatics result showed that a total of 55 biological processes and 39 pathways were significantly enriched(P<0.05).This results partially agreed with the previous studies of YTF in the efficacy and mechanism.Conclusion:Through constructing a multi-dimensional regulatory network based on the network pharmacology,this study initially revealed the mechanism of YTF on CHD and provided a theoretical basis for a futher clinical application.