Sj Dad1反义核酸抗日本血吸虫感染的实验研究

Animal experiment of anti-Schistosoma japonicum with antisense RNA of Sj Dad1

目的 进一步鉴定已获得的日本血吸虫新基因 Ｓｊ Ｄａｄ１的功能。方法 构建Ｓｊ Ｄａｄ１反义（ａｎｔｉｓｅｎｓｅ）核酸载体ＰＥＧＦＰ－Ｎ３－Ｓｊ Ｄａｄ１和正义（ｓｅｎｓｅ）核酸载体 ｐＥＧＦＰ－Ｎ３－Ｓｊ Ｄｅｄ１．ＢＡＬＢ／ｃ小鼠感染日本血吸虫尾蚴后第 ３天，通过小鼠尾静脉分别注射ＰＥＧＦＰ－Ｎ３－Ｓｊ Ｄａｄ１（ａｎｔｉｓｅｎｓｅ）、ｐＥＧＦＰ－Ｎ３－Ｓｊ Ｄａｄｌ（ｓｅｎｓｅ）、空载体和生理盐水。４５ｄ后解剖实验小鼠进行保护性效果评价。结果 在ＰＥＧＦＰ－Ｎ３－Ｓｊ Ｄａｄ１（ａｎｔｉｓｅｎｓｅ）组、ｐＥＧＦＰ－Ｎ３－Ｓｊ Ｄａｄｌ（ｓｅｎｓｅ）组以及ｐＥＧＦＰ－Ｎ３组小鼠肺部冰冻切片检查发现在童虫周围有绿色荧光出现，而在生理盐水组则无此现象。但各实验组保护性效果无统计学差异（Ｐ＞ ０．０５）。结论Ｓｊ Ｄａｄ１反义核酸无明显的抗日本血吸虫感染的效果，尚需寻找其它方法对 Ｓｊ Ｄａｄ１的疫苗候选基因价值作进一步的鉴定。

Aim To learn characters of the Schistosoma japonicum novel gene Sj Dad1. Methods Plasmid pEGFP- N3- Sj Dad1 (antisense) were injected to BALB/c mice through tail vein after Schistosoma japonicum cercariae infection while NS, pEGFP- N3 and pEGFP- N3 - Sj Dad1 (sense) were used as control. Mice were killed after 45 days to check the protective efficacy. Results Fluorescence could be seen around schistosomula in pEGFP-N3 -Sj Dad1 (antisense) pEGFP- N3 and pEGFP- N3- Sj Dad1 (sense), whereas nothing in NS group. Statistical analysis showed that difference between pEGFP-N3 - Sj Dad1 (antisense) group, pEGFP-N3 group, pEGFP- N3-Sj Dad1 (sense) group and NS group is no significant (P> 0. 05). Conclusions The protection efficacy of antisense RNA of Si Dad1 is not significant. More experiments are required to characterize Sj Dad 1 gene.