大肠癌相关新基因SNC73在人上皮肿瘤组织中的表达研究

Expression of noval gene SNC73 in human cancer tissue tested by in situ hybridization

目的 原位检测大肠癌相关新基因SNC73在常见上皮肿瘤中的表达状况 ,探讨该基因表达与上皮肿瘤发生的相互关系。方法 采用cRNA/mRNA原位杂交的方法 ,用体外转录制备的地高辛标记的cRNA探针对大肠癌、胃癌、肝癌、肺癌和乳腺癌组织及同一患者的相应正常组织作表达情况的配对研究。结果 SNC73基因在胃、大肠黏膜的上皮细胞及淋巴细胞呈强阳性表达 ,在其他组织细胞中未见表达 ;在大肠癌和胃癌组织中存在明显的表达降低或缺乏 ;在肝癌、肺癌和乳腺癌及其相应正常组织中都未见表达。结论 SNC73是一个IgA样消化道肿瘤相关基因 。

Objective To detect the expression status of new gene SNC73 in common epithelium cancer tissues and to elucidate the relationship between the expression of SNC73 and the genesis of tumors. Methods Paired tumor and normal tissues from five different types of human cancers were tested by cRNA/mRNA in situ hybridization using the digoxingen labeled probe. Results SNC73 was highly expressed in epithelium cells and lymphocytes of normal colorectal and gastric mucosa, and its expression was significantly lower or even deficient in colorectal and gastric cancer tissues. No expression could be detected both in the normal and cancer tissues of liver, lung or breast. Conclusions SNC73 is a novel gene related to the cancer of digestive tract, and should be further investigated as a candidate of the tumor suppressor gene.