汉族HLA-A等位基因与银屑病患者相关性研究

A study on HLA-A alleles associated with psoriasis in Han Chinese

目的 探讨HLA A等位基因与汉族人银屑病遗传易感性。方法 利用聚合酶链反应 序列特异性引物 (PCR SSP)法 ,对 2 0 0例银屑病患者和 2 0 4例健康人的HLA A等位基因进行检测。结果 HLA A 2 6 0 1 0 5等位基因与汉族人银屑病呈正相关性 (2 0 .2 5 %vs 12 .2 5 % ,RR =1.6 5 ,χ2 =11.76 ,P =0 .0 0 0 6 ,Pc=0 .0 0 6 6 )。HLA A 0 2 0 1 17等位基因与汉族人银屑病呈负相关 (4.2 5 %vs9.80 % ,RR =0 .43 ,χ2 =10 .2 6 ,P =0 .0 0 13,Pc=0 .0 143)。HLA A 2 6 0 1 0 5等位基因仅与有家族史银屑病呈正相关 (RR =2 .0 4,χ2 =12 .49,P =0 .0 0 0 4,Pc=0 .0 0 44 )。HLA A 2 6 0 1 0 5等位基因与Ⅰ型银屑病呈正相关 (RR =1.6 8,χ2 =11.6 7,P =0 .0 0 0 6 ,Pc=0 .0 0 6 6 )。结论 HLA A 2 6 0 1 0 5可能是银屑病的易感基因或与易感基因相连锁。HLA A 2 6 0 1 0 5仅为有家族史银屑病和Ⅰ型银屑病的危险基因。

Objective To explore the potential association of HLA-A alleles and genetic susceptibility to psoriasis (PS) in Han Chinese. Method Primer (PCR-SSP) method polymerase chain reaction sequence specific was used to analyze the distribution of HLA-A alleles among 200 patients with psoriasis and 204 healthy pessons. Results ① HLA-A*2601-05 allele was positively associated with PS (20.25% vs 12.25%, RR=1.65, χ2=11.76, P=0.000?6, P c=0.006?6). HLA-A*0201-17 (4.25% vs 9.80%, RR=0.43, χ2=10.26, P= 0.001?3, P c=0.014?3) allele was negatively associated with PS. ② HLA-A*2601-05 allele was only increased in patients with family history (25.00% vs 12.25%,RR=2.04, χ2=12.49, P=0.000?4,P c=0.004?4). ③ HLA-A*2601-05 (20.61% vs 12.25%, RR=1.68, χ2=11.67,P=0.000?6, P c=0.006?6) allele was only associated with typeⅠpsoriasis. Conclusion ① HLA-A*2601-05 allele may be a susceptible genes or may have close linkage with the susceptible genes. ② HLA-A*2601-05 allele was a risk gene for psoriasis patients with family history and typeⅠpsoriasis. [ Key words ] Psoriasis; HLA-A alleles; Polymerase chain reaction-sequence specific primer P<0.01);3 d later,the activities of 5 enzymes were still increased [(21.3±12.3),(105.8±51.4),(144.8±51.4),(159.8±35.4),(16.2±9.1)U/L respectively].7 and 12 d later,the activities of α-HBDH and CK-MB were still higher than those of control(P<0.01).LDH 1 and LDH 2 increased to peak value in 24 h after poisoning (35.3±5.8),(43.8± 5.7)U/L vs (24.8±3.9),(36.9±4.3)U/L,P<0.01.The abnormal rate of serum LDH 1 was 78.7%,LDH 2 58.3%,LDH 45.2%,CK-MB 37.1%,α-HBDH 33.6% and the abnormal rate of ECG was less than 10%. Conclusion Acute carbon monoxide poisoning may cause myocardial injury.Determination of serum myocardial enzymes may contribute to showing myocardial injury,early diagnosis and treatment,results of treatment and prognosis.