幽门螺杆菌cagA、vacA抗体与胃十二指肠疾病的相关性研究

STUDY ON RELATIVITY BETWEEN HELICOBACTER PYLORI, HP (cagA,vacA) AND THE GASTRODUODENAL DISEASES

目的 :探讨幽门螺杆菌 (Hp)毒力基因cagA、vacA抗体与胃十二指肠疾病之间的关系。方法 :采用免疫印迹法检测 440例胃十二指肠疾病患者血清中的cagA、vacA抗体。结果 :cagA、vacA抗体在 440例患者中的检出率分别为 73 %、37.0 %。在慢性胃炎 (CG)、十二指肠球部溃疡 (DU)、胃癌 (GC)患者中 ,cagA、vacA抗体的阳性率分别为 62 .9% ,76 .1 %、96 .9%与 33 .0 %、31 .0 %、62 .5 % ;经u检验显示 :慢性胃炎组与十二指肠球部溃疡组比较 ,无明显差异。胃癌组与慢性胃炎组、十二指肠球部溃疡组比较 ,有显著性差异。结论 :本文通过患者血清中Hp抗体 (cagA和vacA)的检测 ,推知其cagA和vacA抗体的表达状况 ,可为胃十二指肠疾病的诊断提供依据 。

Objective:In order to find the relationship between the Cytotoxin associated geneA(cagA), Vacuolating Cytotoxin(vacA ) of the Helicobacter Pylori,Hp and Gastroduodenal Disease.Methods:The studied population comprised 440 patients with gastroduodenal diseases.cagA and vacA in serum were measured by immunoblotting. Results:The determinative rates of cagA and vacA in serum were 73.0%, 37.0% respectively. In the patients with chronic gastritis (CG),duodenal ampulla ulcer(DU), gastritis cancer(GC), were 62.9%, 76. 1% and 96.9%, positive rates of vacA were 33.0%, 31.0% and 62.5%.It indicate that CG groups were not different from DU groups,but GC groups were significantly different from DU groups and CG groups.Conclusions: The study suggests that the expressions of cagA and vacA is one of effective methods for diagnosing gastroduodenal diseases. However, it was not used as only one standard for identification of gastroduodenal patient infected with Helicobacter pylori.