日本血吸虫成虫67kDa分子抗原的诊断价值

Evaluation of 67kDa Antigenic Molecule Purified From Adult Worm Antigen of Schistosoma japonicum (SjAwA67)in Immunodiagnosis

为检测日本血吸虫成虫67kDa分子抗原（sjAWA67）对血吸虫病的诊断价值,本研究通过SDS-PAGE和电渗方法,从日本血吸虫成虫抗原中分离纯化出67kDa分子抗原,并用该抗原包被酶标反应板微孔,进行常规ELSA检测。结果对急、慢性血吸虫病患者血清的检出率分别为100％和95％,与正常人血清、肝吸虫病和肺吸虫病患者血清均未出现明显的交叉反应,44例血吸虫病患者治疗后3、6和12个月后的阴转率分别达45.5％、75.0％和90.9％。表明SjAWA67蛋白具有较好的疗效考核价值和现场应用前景。

To evaluate the specificity and sensitivity of SjAWA67 in detecting specific antibodies in sera of patients with schistosomiasis japonicum and in assessing therapeutic efficacy. Methods: A major antigenic protein of 67kDa was purified from adult worm antigen of Schistosoma japonicum (SjAWA) by SDS-PAGE and electric elution. SjAWA67-ELISA was used to determine the specific IgG in sera of healthy individuals, patients with schistosomiasis japonicum at different time after chemotherapy and patients with other parasitic diseases. Results: SjAWA67-ELISA was as sensitive and specific as SjSEA-ELISA. The negative conversion rate of IgG detected by SjAWA67 3 months after treatment was 45.5% , being significantly higher than 31.8% by AWA-ELISAand 11.4% by SjSEA-ELISA respectively, and in addition, the parameter detected by SjAWA67 12 months after treatment was 90. 9% , being significantly higher than 72. 7% by AWA-ELISA and 52. 3% by SjSEA-ELISA respectively. Conclusion: SjAWA67 is a useful antigen for immunodiagnosis of schistosomiasis japonicum.