原发性肝细胞癌中游离脂肪酸受体4的表达及其临床意义

Expression of FFAR4 gene in primary hepatocellular carcinoma and its clinical significance

目的探讨游离脂肪酸受体4(FFAR4)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达及其临床意义。方法采用免疫组化SP法检测102例HCC及其对应癌旁组织中FFAR4表达,并分析FFAR4的表达与HCC临床病理特征之间的相关性。利用qRT-PCR及Western blot法检测20对新鲜冷冻HCC组织及对应癌旁组织中FFAR4的表达,并复习相关文献。结果FFAR4在HCC及癌旁组织中的高表达率分别为64. 7%(66/102)、15. 7%(16/102),两组差异有统计学意义(P <0. 05),FFAR4高表达与HCC肿瘤血管侵犯(P <0. 05)、TNM分期(P <0. 01)及Edmondson分级(P <0. 05)有关。qRT-PCR及Western blot法检测结果均显示HCC组织中FFAR4的表达量显著高于癌旁组织,组间差异有统计学意义(P均<0. 05)。结论FFAR4表达与HCC是否存在血管侵犯、TNM分期以及Edmondson分级均有显著相关性。FFAR4高表达可能与HCC患者病情严重程度密切相关。

Purpose To investigate the expression and clinical significance of free fatty acid receptor 4( FFAR4) in hepatocellular carcinoma( HCC). Methods The expression of FFAR4 in HCC tissues and adjacent tissues of HCC patients was confirmed by 102 cases of liver resection and postoperative pathology,and the relationship between FFAR4 expression and clinical data of HCC patients was analyzed. Quantitative realtime PCR( qRT-PCR) and Western blot were used to detect the expression of FFAR4 in 20 pairs of freshly frozen HCC and adjacent tissues,and the related literatures were reviewed. Results The expression rate of FFAR4 in HCC tissues was 64. 7%( 66/102),and that in adjacent tissues was 15. 7%( 16/102).The difference in FFAR4 expression between the two groups was statistically significant( P < 0. 05). The high expression of FFAR4 in HCC tissues was significantly correlated with tumor vascular invasion( P < 0. 05),TNM stage( P < 0. 01),and Edmondson classification( P < 0. 05). qRT-PCR and Western blot showed that the expression of FFAR4 in HCC tissues was significantly higher than that in adjacent tissues. The difference between the two groups was statistically significant( P < 0. 01,P< 0. 05). Conclusion The expression of FFAR4 is significantly associated with the presence of vascular invasion,TNM staging,and Edmondson grading in HCC. High expression of FFAR4 may be closely related to the severity of HCC patients.