结直肠癌原发灶及肝脏转移灶中TMSG-1的表达及意义

Espression and significance of TMSG-1 expression in primary and liver metastases of colorectal cancer tissues

目的探讨肿瘤转移抑制基因(tumor metastasis suppressor gene-1,TMSG-1)在结直肠癌组织及对应肝脏转移灶中的表达,并分析其与临床病理特征的关系。方法采用免疫组化SP法检测200例结直肠癌原发灶和52例肝脏转移灶中TMSG-1蛋白的表达。结果结直肠癌原发灶中TMSG-1的高表达率为42. 5%(85/200),中等强度表达率为29. 5%(59/200),阴性率为28. 0%(56/200)。结直肠癌肝脏转移灶中TMSG-1的高表达率为17. 3%(9/52),中等强度表达率为50. 0%(26/52),阴性率为32. 7%(17/52)。TMSG-1在结直肠癌原发灶中的表达与肿瘤分化程度、浸润深度、有无淋巴结转移、有无远处转移及肿瘤TNM分期显著相关(P <0. 05),与患者年龄、性别及肿瘤大小无关。TMSG-1在结直肠癌肝脏转移灶中的表达显著低于原发灶(P <0. 05),且在肝脏转移灶中的表达与临床病理特征无明显相关性。结论 TMSG-1在肿瘤转移过程中表达明显降低,其低表达与结直肠肿瘤进展密切相关,有望在病理诊断中作为一种新型肿瘤标志物判断结直肠癌的预后。

Purpose To investigate the expression of tumor metastasis suppressor gene-1( TMSG-1) in colorectal cancer tissues and liver metastases,and to analyses the relationship between expression of TMSG-1 and clinicopathologic characteristics. Methods Immunohistochemical SP methods was used to detect the expression of TMSG-1 protein in 200 cases of colorectal cancer and 52 cases of liver metastases. Results The ratio of high,moderate and negative of TMSG-1 expression in primary colorectal carcinoma were 42. 5%( 85/200),29. 5%( 59/200) and 28. 0%( 56/200) respectively. The expression of TMSG-1 was significantly associated with tumor differentiation,invasion depth,lymph node metastasis,distant metastasis and TNM stages( P < 0. 05),but unrelate to age,gender and tumor size. The high, moderate and negative expression ratio of TMSG-1 in liver metastases were 17. 3%( 9/52),50. 0%( 26/52),32. 7%( 17/52),respectively. The expression of TMSG-1 in liver metastases was significantly lower than that in primary lesion( P < 0. 05). The expression of TMSG-1 in liver metastases,and there was no significant correlation between the expression of TMSG-1 in liver metastases and clinicopathologic characteristics. Conclusion The expression of TMSG-1 is significantly down-regulated in the liver metastases,which is associated with the development and progression of colorectal cancer.TMSG-1 will be used as a new tumor marker for predicting the prognosis of colorectal cancer.