肺癌骨转移患者血清和转移灶中LCN2和PDGF-BB的检测及意义

Detection and significance of LCN2 and PDGF-BB in serum and metastasis of lung cancer patients with bone metastasis

目的探讨脂质运载蛋白2(lipocalin-2,LCN2)和血小板衍生生长因子BB(platelet derived growth factor-BB,PDGF-BB)在肺癌患者血清、癌组织和骨转移灶中的表达。方法应用蛋白芯片筛选19例(9例仅发生骨转移和10例无远处转移)肺癌患者血清中差异表达的细胞因子;采用免疫组化法检测12例无远处转移的肺癌原发灶和12例仅发生骨转移的骨转移灶中LCN2和PDGF-BB因子的表达。结果非小细胞肺癌患者血清中LCN2和PDGF-BB在骨转移组中的表达明显高于无远处转移组(P <0. 05);小细胞肺癌患者血清中未检测到差异表达的细胞因子(P> 0. 05)。免疫组化结果显示LCN2和PDGF-BB在非小细胞肺癌患者骨转移灶中呈高表达,高于肺癌原发灶组织(P <0. 05)。结论 LCN2、PDGF-BB在非小细胞肺癌骨转移患者血清及骨转移灶中高表达,可能在骨髓微环境中参与肺癌骨转移的发生、发展,有望成为肺癌骨转移的重要检测指标和潜在治疗靶点。

Purpose To investigate the expression of lipocalin-2( LCN2) and plateled derived growth factor-BB( PDGF-BB) in serum,carcinoma and bone metastases of lung cancer patients. Methods Protein chip were used to screen the differential expression of cytokines in serum of 19 lung cancer patients( 9 patients with bone metastasis and 10 patients freedistant metastasis). Immunohistochemistry was performed to assess the differential expression of LCN2 and PDGF-BB cytokines in 12 cases of primary lung cancer without distant metastasis and12 cases of primary lung cancer with only bone metastasis. Results Serum level of lipid transport factor( LCN2) and PDGFBB in non-small cell lung cancer patients with bone metastasis were significantly higher than that without distant metastasis( P< 0. 05). There was no difference cytokines between small cell lung cancer patients with bone metastasis and without metastasis group( P > 0. 05). The results of immunohistochemistry showed that high expression of LCN2 and PDGF-BB in bone metastasis tissues was significantly higher than that in primary lung cancer tissues. Conclusions High expression of LCN2 and PDGF-BB in serum and bone metastasis tissue of patients with non-small cell lung cancer might be involved in the occurrence,development of bone metastasis of lung cancer in the bone marrow,may be an important biomarker and potential therapeutic target for bone metastasis of lung cancer.