整合素β_1在下颌骨牵张成骨过程中的表达研究

A study of integrinβ_1 expression during the distraction osteogenesis of mandible

目的:探讨下颌骨牵张成骨过程中整合素β1的表达变化。方法:作犬下颌骨颊舌向骨皮质牵张模型。固定牵张器一周后开始牵张,每天2次,每次0.5mm,连续7天。牵张完成后第4天及第1、2、4、6、8周取新生骨组织标本作成石蜡切片并用LSAB法进行免疫组化检测,观察整合素β1在新骨组织,特别是在成骨细胞和破骨细胞上的表达。结果:牵张成骨过程中,整合素β1在新骨的成骨细胞与破骨细胞的表达不同。成骨细胞膜上整合素β1在第一周开始表达,第2-4周表达最强,第8周恢复正常水平。在破骨细胞膜上第4天弱阳性表达。一周后数目明显抑制,第4周消失。结论:牵张成骨过程中,成骨细胞整合素β1表达增强,提示在牵张成骨中发挥重要作用,整合素β1可能与介导应力信号转导有关。

Objective:To investigate the expressing changes of integrin β1 during the distraction osteogenesis ofmandible.Methods:Canine mandible was used as buccolingular distraction model.The bone segment was elongated buccally after DO device applied one week.The distraction protocol consisted of a rate of 0.5mm twice daily for 7 days,followed by a consolidation phase of 7 days.The newly osteogenesis were obtained and paraffin slices were maderespectively on the 4th day,the lst.2nd,4th,6th,8th week after distraction complete.The expression of integrin β1 in bone tissue,especially in the membrane of osteoblast and osteoclast was detected with LSAB immunohistochemical method.Results:Integrin β1 was expressed differently in osteoblast and osteoclast at the process of distraction.In osteoblasts,integrin β1 expressed in creased on the lst week,maximum on the 2nd-4th week and normal on the 8th week.While in osteoclast,inferror positive expression of integrin β1 was observed on the 4th day,decreased on the lst week and disqppeared on the 4th week.Conclusion:During distraction osteogengsis,the increased expression of integrin β1 in osteoblast suggested that integrin β1 has a critical in fluence on distraction osteogenesis,and likely relates to transduct the mechanical signal.