小儿病毒性心肌炎红细胞膜流动性的变化

Fluidity Change of Erythrocyte Membrane in Children with Acute Viral Myocarditis

本文测定了20例急性病毒性心肌炎患儿治疗前后红细胞膜微粘度及血浆脂质过氧化物,并以20名同龄健康小儿作对照。治疗前红细胞膜粘度和血浆脂质过氧化物水平明显增高(P<0.01)。二变量间呈有意义的正相关(r=0.88,P<0.01)。治疗后红细胞膜微粘度和血浆脂质过氧化物都接近正常对照组水平(P>0.05)。二者治疗前后差异非常显著(P<0.01)。提示红细胞膜流动性降低可能在小儿病毒性心肌炎发病机制中起一定的作用。

The fluidity of erythrocyte membrane in 20 children with acute viral myocarditis was determined by fluorescence polarization technique using 1, 6-diphenyl-1, 3, 5-hexatriene (DPH) as fluorescence probe before and after treatment. Plasma lipid peroxide (LPO) was determined in the meantime. The results showed that before treatment plasma LPO and erythrocyte membrane micro-viscosity (EMMV)in patients were higher than that in normal controls (n=20). The values were 3.88±0.71 nmol/ml and 3.73±0.61 respectively, while that in controls were 1.98±0.49nmol/ml and 2.50±0.36 respectively (both P<0.01). After using scavengers (Danshen and VC、VE) for 4-6 weeks plasma LPO and EMMV of patients significantly decreased. The values were 2.28±0.56nmol/ml and 2.71±0.54 respectively (both P>0.05 compared with normal controls). The results suggested that plasma LPO may have an effect on erythrocyte membrane fluidity. It was concluded that lower fluidity of erythrocyte membrane may play an important role in the pathogenesis of AVMC.