HLA-C基因分型与皖籍汉族人寻常性银屑病关系的研究

HLA-C Genotyping in Patients with Psoriasis Vulgaris in Anhui Han Population

目的 探讨皖籍汉族人寻常性银屑病 (PsV )与HLA C等位基因的相关性。方法 运用聚合酶链反应 -序列特异性引物 (PCR SSP)法 ,对 166例皖籍PsV患者和 2 48例健康对照进行HLA C基因分型。结果 ①PsV患者组HLA Cw 0 60 2等位基因频率较对照组显著升高 (OR =4.13 ,95 %可信限 2 .3 8～ 7.16,Pc <0 .0 1) ,但HLA Cw 0 3 0 4等位基因频率则较对照组明显减低 (OR =0 .3 2 ,95 %可信限 0 .17～ 0 .60 ,Pc <0 .0 1) ;②早发型PsV (I型银屑病 )及有PsV家族史患者携带HLA Cw 0 60 2等位基因频率分别高于迟发型PsV (II型银屑病 )及无PsV家族史患者 (OR =4.3 2 ,95 %可信限 2 .44～ 7.67,Pc <0 .0 1和OR =13 .2 8,95 %可信限 6.12～ 2 9.11,Pc <0 .0 1)。结论 皖籍汉族人PsV与HLA Cw 0 60 2等位基因高度关联 ,且携带该等位基因的个体易发生早发型银屑病 ,并有家族倾向性。

Objective To study the association of HLA-C alleles with psoriasis vulgaris(PsV) in Anhui Han population.Methods Using PCR-SSP (polymerase chain reaction-sequence specific primers), we detected the distributing frequencies of HLA-C alleles among 166 patients with PsV and 248 healthy controls from Anhui province.Results ① HLA-Cw*0602 allelic frequency increased in patients with PsV compared with that in control population(OR=4.13,95% confidence limit 2.38～ 7.16,Pc<0.01),while HLA-Cw*0304 allelic frequency was detected in patient with PsV compared with that in control population(OR=0.32,95% confidence limit 0.17～0.60,Pc<0.01).②The distributing frequency of HLA-Cw*0602 allele increased in patients with early-onset(type I) psoriasis and those from psoriatic family,but did not in those with late-onset(type II) psoriasis and those from non-psoriatic family(OR=4.32,95% confidence limit 2.44～7.67,Pc<0.01 and OR=13.28,95% confidence limit 6.12～29.11,Pc<0.01).Conclusion HLA-Cw*0602 allele was associated with PsV in Anhui Han population,and these patients carrying HLA-Cw0602 allele was easier to develop early-onset psoriasis.