摘要
目的 用经动物血浆处理后冷存的异体神经移植体,缝接神经缺损,提高神经再生。方法 用15只兔左、右肢正中神经30条,分别切除2.5 cm为实验材料,动物分两组:A组15条神经用经受体动物血浆浸泡冷冻处理的异体神经移植为实验组,B组15条神经只经冷冻处理的异体神经缝接,术后不同时间,采用光、电镜组织学,图像分析仪测定,神经外周粘连定量测定和电生理指标等观察。结果(1)A组再生神经纤维在数量上和直径上均明显较B组的多而粗(p<0.03);(2)A组神经缝合段的结缔组织增生和与其外周组织粘连程度均较B组轻(p<0.01)。结论 经受体血浆处理后冷存的异体神经移植体,有提高神经再生的作用。
Objective To study the effect of pre-freezing treatment with immersed heterogenous transplant in recipient blood plasma on regeneration of allogenic nerve graft. Methods A total of 30 left and right median nerves were excised by 2.5 cm, and the excised nerves were anastomosed with hetero-median nerve. Animals were divided into two groups. In group A 15 median nerves were injured. All the excised nerves were immersed in receptor blood plasma for 20 minutes, then were only stored in - 196℃liquid nitrogen for 21 days; after the excised nerves recovered to room temperature. In group B 15 median nerves were injured, all the excised nerves were only stored in - 196℃ liquid nitrogen for 21 days; after the excised nerves recovered to room temperature. In the different time after operation, microscopic observation, Luzex-F image pattern analysis instrument determination, quantitative determination of adherent amount around nerve and electro-physiological index examination were analysed. Results (1) The myelinated nerve fiber in neural transplant segment and the distal segment of A group were more and thicker than the B group in both number and diameter(p< 0.05) . (2) Proximal suture region of A group was less adhesion to peripheral tissue than B group (P< 0.01) . Conclusion Heterogenous transplant in recipient animal plasma is ability to accelerate the neuron regeneration.
