IL-2对人胃癌细胞侵袭力及转移力的调节作用

In vitro modulation of the invasive and metastatic potentials of human gastric cancer by interlukin-2

目的 研究IL 2对胃癌细胞侵袭力及转移力相关因素的调节作用。方法 用流式细胞仪分析胃癌细胞表面抗原分子的表达水平 ,用附壁试验检测胃癌细胞对细胞外基质的亲和力 ,用细胞聚集试验检测胃癌细胞的聚集能力。结果 经高浓度和低浓度IL 2处理后 ,胃癌细胞表面ICAM 1及HLA 1表达均增加 ,CD4 4表达减少 ,胃癌细胞对细胞外基质的亲和力及胃癌细胞聚集程度均降低。结论 IL 2可明显增加胃癌细胞ICAM 1及HLA 1的表达 ,抑制CD4 4表达 ,抑制胃癌细胞对细胞外基质的亲和力及胃癌细胞的聚集作用。因此可抑制胃癌细胞的侵袭力和转移力。

Objective To investigate the effects of Interlukin 2(IL 2)on the in vitro invasiveness and the expression of several cell surface antigens related to invasive and metastatic potentials of human gastric cancer SGC 7901 cell line. Methods The expression of ICAM 1, CD44 and HLA 1 was determined by fluorescence activated cell sorter (FACS) analysis, the tumor cell binding affinity to extracellular matrix (ECM) components was measured by cell attachment assay, the degree of homotypic aggregation was quantified by cell aggregation assay. Results The results showed that IL 2 pretreatment exhibited enhanced expression of ICAM 1 and HLA 1, suppression of CD44 on gastric cancer cell line and decreased binding affinity to ECM components and the degree of homotypic aggregation of gastric cancer cells. Conclusion It suggested that IL 2 can inhibit the invasive and metastatic potentials of gastric cancer cells.