免疫球蛋白重链T细胞受体γ基因重排在急性淋巴细胞白血病中的表达

Rearrangements of Immunoglobulin Heavy Chain and T Cell Receptor γ Gene in Acute Lymphocytic Leukemia

目的 :探讨初诊急性淋巴细胞白血病 (ALL)患者免疫球蛋白重链 (IgH)、T细胞受体γ(TCRγ)基因重排情况。方法 :应用多聚酶链反应 (PCR)检测 19例初发ALL患者骨髓IgH、TCRγ基因重排。结果 :19例标本中 10例发生单一IgH基因重排 ,5例发生单一TCRγ基因重排 ,2例同时出现IgH/TCRγ基因重排。结论 :ALL患者IgH基因重排几率高于TCRγ基因重排 ,且存在着交叉重排现象。

Objective:To explore the occurrence rate of rearrangements of immunoglobulin heavy chain (IgH) and T cell receptor γ (TCRγ) genes in the patients with acute lymphocytic leukemia (ALL). Methods: Polymerase chain reaction (PCR) was used to detect IgH and TCRγ gene rearrangements in the bone marrow samples from 19 ALL patients. Results:IgH gene rearrangements were found in 10 patients,and TCRγ gene rearrangements in 5 patients; 2 cases had both gene rearrangements. Conclusion:In ALL,the occurrence of IgH gene rearrangements was higher than that of TCRγ gene rearrangements, and there was the phenomenon of cross rearrangements.