反复热性惊厥对大鼠学习记忆的影响及其机制

Effect of repeated febrile convulsions on memory in rats and its possible mechanism

目的 观察热性惊厥对学习记忆的影响及其与海马苔藓纤维发芽的关系。方法 采用热水浴诱导惊厥模型 ,诱导生后 2 2dSD大鼠发生 15次热性惊厥。采用Morris水迷宫方法检测大鼠惊厥后近期和远期 (3个月后 )空间学习记忆能力 ;采用Timm染色观察海马颗粒细胞苔藓纤维发芽现象。结果 热性惊厥组大鼠在惊厥后早期和 3个月后的寻找平台潜伏期均较对照组明显延长。Timm染色显示惊厥 15次大鼠海马区有明显的苔藓纤维发芽现象 ,持续至 3个月后。结论 反复热性惊厥可造成学习记忆损伤 。

Objective To elucidate the effect of febrile convulsions (FCs) on memory ability and its relationship to hippocampal mossy fiber sprouting.Methods Warm water immersion rat FC model was developed. From 22 days of age, the SD rats were induced seizures 15 times. The memory ability was tested using a Morris water maze method after seizures and an interval of 3 months. Mossy fiber sprouting was detected by Timm stain.Results Rats with FCs performed significantly worse in the Morris water maze both after seizures and 3 months later. Significant mossy fiber sprouting phenomenon was detected after repeated FCs, persisting at least 3 months.Conclusion Repeated FCs resulted in memory deficit and mossy fiber sprouting may underlie the effect of FCs.