氯和二氧化氯灭活甲型肝炎病毒机理的研究

STUDY ON MECHANISM OF INACTIVING HEPATITIS A VIRUS BY CHLO-RINE AND CHLORINE DIOXIDE

为探讨氯和二氧化氯灭活HAV机理及用PCR评价消毒效果的可行性,采用细胞培养、ELISA及大片段逐步步移RT-PCR方法对消毒前后HAV感染性、HAAg抗原性及HAV核酸全序列进行检测。结果,以含有效氯10 mg/L消毒液作用30 min,或含二氧化氯7.5 mg/L的消毒液作用10 min,对HAV感染性的灭活率均为100％,均可破坏HAV核酸5’非编码区;但检测两者HAAg抗原性P/N值分别为3.21(阳性)与2.02(阴性)。结果表明,HAV感染性的灭活与HAV核酸5’非编码区破坏是一致的,可用PCR技术检测HAV核酸5’非编码区以判定HAV灭活与否。

In order to explore the mechanism of inactivating HA V by chlorine and chlorine dioxide and the feasibility of evaluating the disinfection efficacy by PCR, the HAV infectivity, HAAg antigenicity and whole sequence of HAV RNA were examined before and after disinfection using the methods of cell culture, ELISA and big-fragment stepwise walking RT-PCR. The results showed that the disinfectant solution containing available chlorine 10 mg/ L with a 30 min contact time or the solution con taining chlorine dioxide 7.5 mg/ L with a 10 min contact time could inactivate 100% of HAV infectivity and destroy 5' non-coding area of HAV RNA, but their P/ N value for HAAg antigenicity was 3.21 (positive) and 2.02 (negative) respectively. The results indicate that inactivation of HAV infectivity conforms to destruction of 5' non-coding area of HAV RNA and PCR technique can be used for examining 5'non-coding area of HAV RNA in order to judge inactivation of HAV or not.