两种试验方法检测臭氧水杀菌效果的比较

COMPARISON OF EXAMINATION OF GERMICIDAL EFFICACY OF OZONE WATER BY TWO TEST METHODS

用悬液定量杀菌试验法和消毒液流动浸泡载体定量杀菌试验法比较了臭氧水对大肠杆菌杀灭效果及蛋白胨对其杀菌效果的影响。结果,用悬液定量杀菌试验法在不含蛋白胨的条件下,含臭氧8.0 mg/L的臭氧水对大肠杆菌作用1 mim,平均杀灭率为100％;含蛋白胨10 g/L时,作用10 min,对大肠杆菌平均杀灭率为65.96％。用消毒液流动浸泡载体定量杀菌试验法,含臭氧8.0 mg/L的臭氧水作用10 min,对大肠杆菌平均杀灭率为99.97％;菌悬液中含小牛血清体积分数25％以上,对其杀菌效果有明显影响。结果提示,生产流动状态的臭氧水用于表面消毒时,以消毒液流动浸泡载体定量杀菌试验法检测其杀菌效果比较接近实际。

Suspension quantitative germicidal test method and quantitative germicidal test method using carriers immersed in running liquid disinfectant for examining efficacy of ozone water in killing Escherichia coli were compared and the influence of peptone on its germicidal efficacy was examined. The results indicated that when suspension quantitative germicidal test method was used, in absence of peptone, the ozone water containing ozone 8.0 mg/ L with a 1 min contact time killed 100% of E. coli in average and if peptone 10 g / L was present, the same ozone water with a 10 min contact time killed 65.96% of E. coli in average. When quantitative germicidal test method using carriers immersed in running liquid disinfectant was used, the ozone water containing ozone 8.0 mg/ L with a 10 min contact time killed 99.97% of E. coli in average and if the bacterial suspension contained higher than 25% volume of calf serum, the germicidal efficacy was influenced significantly. The results suggest that when running ozone water is used in surface disinfection, examination of germicidal efficacy by quantitative germicidal test method using carriers immersed in running liquid disinfectant relatively approximates the real condition.