元首口服液的二苯乙烯甙含量与O·_2~—清除率关系的实验研究

Experimental study on relationship between content of diphenyl-vinyl-glucosidein Yuanshou Oral Liquid and clearance rate of O·_2^-

目的 探讨元首口服液有效成分二苯乙烯甙含量与其对超氧阴离子自由基 (O·-2 )清除率的关系。方法 紫外分光光度法测定样品中二苯乙烯甙含量 ,用AP -TEMED比色测定样品中O·-2 含量 ,计算其对O·-2 的清除率。结果 一组二苯乙烯甙含量和O·-2 清除率 (X)分别是 0 .2 76 %和 5 4 .5 % ,二组分别为 0 .177%和 2 8.8%。两组二苯乙烯甙含量比较P <0 .0 1;O·-2清除率比较 ,P <0 .0 5 ,R =0 .6 118,直线回归方程为Y =0 .2 893+3.16 0 9X ,P <0 .0 1。结论随着二苯乙烯甙含量升高 ,对O·-2 清除率升高 。

Objective It is to study the relationship between the content of diphenyl vinyl glucoside in Yuanshou Oral Liquid and the clearance rate of O·-2. Methods The content of diphenyl vinyl glucoside in Yuanshou Oral Liquid was detected by ultraviolet spectrophotometry and the content of O·-2 was detected by AP TEMED colorimetric determination. Then clearance rate of O·-2 was calculated. Results The content of diphenyl vinyl glucoside and the clearance rate of O·-2 in group 1 was 0.276% and 5.45% respectively. Those in group 2 were 0.177% and 28.8% respectively. There was significant difference between two groups. Conclusion The content of diphenyl vinyl glucoside is positively related to the clearance rate increasing of O·-2.