Vimentin和Suivivin在子宫内膜腺癌中的表达及其相关性研究

Expression and Its Correlation of Vimentin and Suivivin in Human Endometrial Adenocarcinoma

目的探讨Vimentin和Suivivin在子宫内膜不典型组织及子宫内膜腺癌中的表达及意义。方法收集我院2009年至2012年刮宫或者手术切除的存档石蜡标本,根据组织类型分为两组:子宫内膜不典型增生组和子宫内膜腺癌组,应用免疫组化技术研究Vimentin和Suivivin的表达情况。结果 Vimentin和Suivivin在两组中的阳性表达位于细胞质内,Vimentin和Suivivin在子宫内膜腺癌组中的表达均显著高于子宫内膜不典型增生组(P<0.05)。与子宫内膜不典型增生组相比,子宫内膜腺癌的Vimentin表达增高(P<0.05),其中Vimentin表达与子宫内膜腺癌临床分期及淋巴结转移正相关(P<0.05)。与子宫内膜不典型增生组相比,Suivivin在子宫内膜腺癌中异常表达(P<0.05),且Suivivin异常表达和Vimentin阳性表达呈正相关,与子宫内膜腺癌的5 a生存率呈正相关。结论Vimentin和Suivivin共同参与子宫内膜腺癌的恶性转化过程,联合检测Vimentin和Suivivin对于早期判断子宫内膜腺癌的发生、发展及预后有一定价值。

Objective To investigate the expression and significance of Vimentin and Suivivin in human endometrial atypical hyperplasia and endometrial adenocarcinoma. Methods The archived paraffin specimens were collected from the First Affiliated Hospital of Henan University of Science and Technology from 2009 to 2012. Samples were divided into two groups according to tissue type: endometrial atypical hyperplasia group and endometrial adenocarcinoma group. The Vimentin and Suivivin protein were detected by immunohistochemistry technique. Results The positive expression of Vimentin and Suivinin in both groups were located in the cytoplasm. The expression of Vimentin and Suivivin in intrauterine membrane adenocarcinoma examples was significantly higher than that in endometrial atypical hyperplasia (both P <0.05). Compared with endometrial atypical hyperplasia,expression of Vimentin increased in endometrial adenocarcinoma. Moreover,in endometrial adenocarcinoma group,the expression of Vimentin was correlated with the clinical stage and lymph node metastasis (P < 0. 05). Compared with endometrial atypical hyperplasia,Suivivin was abnormally expressed in endometrial adenocarcinoma (P<0.05).Furthermore,the Suivivin expression was significantly positive correlation with the Vimentin expression. And the Suivivin expression was positively related to 5-year survival rate of the endometrial adenocarcinoma.Conclusion Vimentin and Suivivin are involved in the malignant transformation of endometrial adenocarcinoma. The combined detection of Vimentin and Suivivin is valuable for the early diagnosis of endometrial adenocarcinoma and its occurrence,development and prognosis.