EXPRESSION DIFFERENCES OF VASCULAR GROWTH FACTORS IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549 AND CISPLATIN-RESISTANT HUMAN LUNG ADENOCARCINOMA CELL LINE A_(549)^(DDP)

Objective:To elucidate the expression differences of vascular growth factors in human lung adenocarcinoma cell line A549 and cisplatin-resistant human lung adenocarcinoma cell line A549^DDP.Methods:RT-PCR and immunohistochemistry was used to detect the mRNA and protein expressions of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor (bFGF) in A549 and A549^DDP.Results:VEGF and bFGF mRNA were expressed in A549 and in A549^DDP.VEGF and bFGF mRNA expression levels in A549^DDP were signifi-cant higher than those in A549(P<0.025).VEGF and bFGF protein expressions were all strong positive in A549 and A549^DDP.Conclusion:There are certain differences between VEGF and bFGF expressions in A549 and A549^DDP.Drug-resistance of lung cancer is associated with those above genes over-expressions.Over-expression of vascular growth factors are related to drug resistance of lung cancer.