土壤脲酶和脱氢酶对活性X-3B红污染暴露的耐受性及机理研究

Endurance and Mechanisms of Soil Urease and Dehydrogenase Exposed to Reactive X-3B Red Dye Contamination

采用微宇宙实验方法 ,研究了棕壤、褐土、红壤等 3种我国典型土壤中脲酶和脱氢酶对染料活性X 3B红污染的耐受性及其机理 .实验结果表明 ,3种土壤中的脲酶和脱氢酶活性在试验较低浓度的活性X 3B红污染的环境中均被促进 ,但被促进的程度有所差异 ,具体表现为棕壤脲酶活性被促进的效果最为明显 ,其次红壤 ,褐土最弱 ;较低浓度的活性X 3B红污染对褐土脱氢酶活性的促进作用最强 ,其次红壤 ,棕壤最弱 .3种土壤的脲酶和脱氢酶活性被促进的效果随着时间的推移逐渐减弱 .3种土壤的脲酶和脱氢酶活性对高浓度的活性X 3B红污染并没有显示受抑制的趋势 .相反 ,二者基本维持在未受染料活性X 3B红污染的对照土壤的活性水平 .因此可以初步认为 ,土壤脲酶和脱氢酶对染料活性X 3B红的污染暴露毒性有耐受作用 .基于实验结果 ,还就 2种酶对活性X

Endurance and mechanisms of urease and dehydrogenase in three Chinese typical soils (burozom, cinnamon soil and red soil) exposed to the contamination of reactive X 3B red dye were experimentally studied using the microcosmic method. The results indicated that the activities of urease and dehydrogenase were both promoted in the three soils by reactive X 3B red dye in low dose, 500 and 1000 mg/L although the stimulating intensity was different from each other. For one thing, the activity of urease in burozom was promoted mostly followed by that in red soil and that in cinnamon was promoted most weakly. For the other, reactive X 3B red dye in the same concentration could increase the activity of dehydrogenase in cinnamon soil most greatly and in burozom most weakly. However, the stimulating intensity of urease and dehydrogenase activities in the three soils was more and more fainter with the extension of days. Furthermore, reactive X 3B red dye in high concentration (3000, 5000 and 10000 mg/L) could not inhibit the activities of urease and dehydrogenase in tested soils. On the contrary, all of them remained the level of the activities in unpolluted soils and no toxicity of reactive X 3B red dye could reveal with time. Therefore, both enzymes in three types of soils had the tolerance to reactive X 3B red dye. Based on the experimental data, relevant endurant mechanisms were also analyzed and discussed.