丁二腈高效降解菌的筛选及其降解性

Screening of High Efficiency Succinonitrile Degrading Bacterium Strains and Their Degradability

以丁二腈为唯一碳源和氮源 ,从石化腈纶废水及其处理构筑物的生物膜中 ,分离、筛选出 2株高效降解丁二腈的菌株 :J 1 3和J 1 3 1 .经形态学观察和生理生化特征研究 ,两者均被鉴定为假单胞菌 (Pseudomonasspp .) .通过摇瓶试验得出 2菌株的最适生长条件 :温度为 3 0℃ ,摇床转速 (间接反映通气量 )为 2 5 0r/min ,接种量为0 1 % ,初始pH为 6.在最适生长条件下 ,分别对不同初始浓度丁二腈进行降解率试验 .结果表明 ,2菌株对丁二腈的降解能力强 ,尤以J 1 3 1更为显著 .当丁二腈的初始浓度约为 60 0 0mg/L、80 0 0mg/L和 1 0 0 0 0mg/L时 ,J 1 3 1菌株对丁二腈的降解率分别在 1 2 5h、1 4h和 1 6h时达到 1 0 0 % .

Succinonitrile used as a sole source of carbon and nitrogen,two bacterium strains,named as J 1 3 and J 13 1,which had high degrading capacity for succinonitrile were isolated and screened out from acrylic fiber wastewater and biofilm in its treatment structure. Through morphological and biochemical method,the two strains were primarily identified as Pseudomonas spp. By tests in shaking flasks,it was determined that the strains can be optimum growth at 30℃,with shaker rotary speed which indirectly reflected aeration capacity at 250r/min,inoculum amount of 0.1%, and initial pH6. On the optimum conditions for growth,the degradation experiments on different initial concentrations of succinonitrile were carried out. The results indicate that the two strains,especially J 13 1 had high degrading efficiency for succinonitrile. With the initial concentration of succinonitrile at ca. 6000,8000 and 10000mg/L,the degrading rates of succinonitrile by strain J 13 1 reached to 100% after 12 5h,14h and 16h,respectively.