针刺介入时机对脑梗死大鼠能量代谢和脑源性神经生长因子、酪氨酸激酶受体B的影响

Effect of acupuncture intervention time on energy metabolism and BDNF,TrKB in rats with cerebral infarction

目的研究针刺介入时机对脑梗死大鼠能量代谢和脑源性神经生长因子(BDNF)、酪氨酸激酶受体B(TrKB)的影响,探讨针刺治疗脑梗死的机制及介入时机。方法采用线栓法制备大鼠局灶性永久性脑缺血模型,分为假手术组、模型组、2 h针刺组、3 d针刺组和7 d针刺组5组,在术后1、3、7、14 d,4个时间点分别测定大鼠缺血脑组织钠,钾-三磷酸腺苷酶(Na+,K+-ATP酶)、钙,镁-三磷酸腺苷酶(Ca2+,Mg2+-ATP酶)活性和BDNF、TrKB阳性神经细胞数。结果脑梗死后大鼠脑组织的Na+,K+-ATP酶、Ca2+,Mg2+-ATP酶活性急剧下降,1 d时最低,随着时间的延长出现先升再降的变化。针刺组的ATP酶活性在不同时间点较模型组均有不同程度升高(均P<0.01),2 h组高于3 d和7 d组(P<0.01,P<0.05)。脑梗死后大鼠脑组织中的BDNF,TrKB阳性神经细胞数随着时间延长逐渐增多,各时间点比较差异有统计学意义(P<0.05)。针刺各组的BDNF、TrKB阳性神经细胞数随着时间的延长逐渐增多,各时间点之间比较差异有统计学意义(P<0.01,P<0.05)。2 h针刺组的BDNF、TrKB阳性细胞数比3 d、7 d针刺组的增多更显著,差异有统计学意义(P<0.01,P<0.05)。结论针刺可显著改善缺血脑组织的能量代谢,增加缺血脑组织的BDNF,TrKB阳性神经细胞数量,对脑梗死引起的缺血性脑损伤起修复和保护作用,在脑梗死后越早进行针刺治疗疗效越好。

Objective To research the effects of acupuncture intervention time on energy metabolism and the expression of BDNF and TrKB in rats after ischemic stroke and explore the mechanism of acupuncture treatment and intervention time .Methods The method of permanent focal cerebral ischemia of middle cerebral artery was estab -lished in rats.Two hundreds of model were selected and divided into 5 groups:sham-operation group,model-group,2 h acupuncture group,3 d acupuncture group,7 d acupuncture group.The sodium,potassium-adenosine triphosphatase (Na+,K+ATPase),calcium and magnesium-adenosine triphosphatase (Ca2+,Mg2+ATPase) and the number of BDNF,TrKB positive nerve cells were measured at 1 d,3 d,7 d and 14 d of postoperation .Results The Na+,K+-ATPase,Ca2+,Mg2+-ATPase activity in brain tissue of rats were sharply decreased after ischemia ,lowest at 1d,along with the changes in the extension first and then drop time .ATPase activity in acupuncture group increased significantly in 1,3,7,14 d than those in the model group (P<0.01,P<0.01).2 h group was higher than 3 d and 7 d groups (P<0.01,P<0.05),the difference was statistically significant.The BDNF,TrKB positive nerve cells were gradually increased with time(P<0.05).The BDNF,TrKB positive neural cells of each acupuncture group were increased gradually with time(P<0.01,P<0.05).The number of TrKB positive cells in 2h acupuncture group was higher than that in 3 d,7 d acupuncture group(P<0.01,P<0.05).Conclusion Acupuncture can improve energy metabolism of ischemic brain tissue ,increase the positive neurons cell number of BDNF and TrKB ,repair and protecte the brain injury of ischemic ,the better therapeutic effect the earlier acupuncture treatment .