类风湿关节炎患者血清α-微管蛋白的检测及与临床实验室指标的相关性分析

The detection and correlation research of α-tubulin and disease activity parameters in rheumatoid arthritis

目的利用酶联免疫吸附方法(ELISA)测定类风湿关节炎(RA)患者血清中的α-微管蛋白(α-tubulin)的水平,评价与临床实验室指标的关系,为寻找RA新的血清学标记物,探讨疾病的发病机制提供依据。方法选取RA患者68例,骨关节炎(OA)患者32例,健康对照(HC)32例,各取血清10μL,应用ELISA方法检测不同患者及健康对照血清α-tubulin水平,应用非配对资料的t检验分析不同患者与健康对照者之间α-tubulin水平的差异,相关性检验分析α-tubulin水平与RA患者年龄、病程、压痛关节数、肿胀关节数、红细胞沉降率(ESR)、C反应蛋白(CRP)水平、DAS28评分、类风湿因子(RF)水平、免疫球蛋白(Ig G、Ig A、Ig M)水平的相关性。结果 RA患者血清α-tubulin的水平明显高于骨关节炎患者及健康对照(P<0.05),进一步分析提示α-tubulin水平与RA患者病程长短呈正相关(r=0.307,P=0.023),与血清类风湿因子水平及Ig A水平呈正相关(r=0.558,P=0.0001;r=0.395,P=0.004)。结论α-tubulin是RA新的血清学标记物,在RA患者的免疫反应中具有一定的意义,可能参与了RA的发病。

Objective To detect serum α- tubulin in patients with rheumatoid arthritis( RA) and analyze the correlation between serum α- tubulin and clinical,laboratory parameters of RA,provided the theoretical basis to seek the pathogenesis of diseases. Methods Blood samples from 68 patients with RA,32 patients with Osteoarthritis( OA)and 32 healthy controls were collected. Serum α- tubulin were detected by enzyme-linked immunosorbent assay( ELISA). Difference of α- tubulin between RA patients,OA patients and healthy controls was analyzed by independent sample t test. Correlation analysis was done between α- tubulin level and clinical,laboratory parameters of RA,including age,disease duration,number of tender joints,number of swelling joints,DAS28 score and levels of ESR,C-reactive protein( CRP),Rheumatoid Factor( RF),immunoglobin G( Ig G),immunoglobin A( Ig A) and immunoglobin M( Ig M). Results The α- tubulin of RAwas obviously higher than that of OA patients and healthy controls( P < 0.01). Further analysis demonstrated that α- tubulin level was positively correlated with the disease duration( r = 0. 307,P = 0. 023),levels of RF and Ig A( r = 0. 558,P = 0. 0001; r = 0. 395,P = 0. 004). Conclusion α- tubulin may play roles in the development o f RA and may be a potential biomarker of of RA.