尿液神经丝蛋白检测对老年阿尔茨海默病患者的临床意义

Clinical significance of urinary AD7c-NTP detection in elderly patients with Alzheimer's disease

目的探讨老年阿尔茨海默病(AD)患者尿液中神经丝蛋白含量检测的临床意义。方法 AD组:门诊以及住院诊断为AD患者66例(AD组),其中男性60例,女性6例;年龄范围75～96岁,年龄(87. 27±4. 95)岁;对照组:智能正常老人85名,其中男性72名,女性13名;年龄范围74～98岁,年龄(86. 93±5. 21)岁,所有受试者进行认知功能测评,包括简易智能状态检查量表(MMSE)、临床痴呆评定量表(CDR),Hachinski缺血指数量表(HIS)。根据量表评分将AD组分为轻、中、重组。采集尿液标本进行尿液中神经丝蛋白(AD7c-NTP)含量检测。结果 (1) AD组尿AD7c-NTP阳性明显高于对照组,两组阳性率比较,差异有统计学意义(P <0. 001);(2)两组受试者年龄、性别、教育背景与AD7c-NTP含量的相关性分析(Pearson相关分析)显示,两组尿AD7c-NTP含量与以上诸因素均无明显相关性; MMSE分数与AD7c-NTP含量呈负相关,CDR分数与AD7c-NTP含量呈正相关。(3)轻度AD组与中、重度AD组尿AD7c-NTP含量比较差异有统计学意义(F=88. 756,P <0. 001),提示尿中的AD7c-NTP含量随疾病严重程度增加而上升。结论通过对老年AD患者尿液中AD7c-NTP含量检测,可以对AD的诊断提供帮助,并可能与病情严重程度有一定的关系。

Objective To investigate the clinical value of the of neuronal thread protein( AD7c-NTP) in urine of elderly patients with Alzheimer’s disease. Methods AD group: Outpatients and hospitalized patients were diagnosed as AD patients 66 cases( AD group),including 60 males and 6 females;aged 75 to 96 years,mean age( 87. 27 ± 4. 95)years old;control group: normal elderly 85 Names,including 72 males and 13 females;ages 74 to 98 years,mean age( 86. 93 ± 5. 21) years,all participants assessed cognitive function assessment,including Mini-Mental State Examination( MMSE),clinical dementia Scale( CDR),Hachinski Ischemic Index( HIS). According to the scale score,the AD group was divided into mild,moderate,and severe. Urine samples were collected for urinary levels of AD7c-NTP. Results 1. The positive rate of urinary AD7c-NTP in AD group was significantly higher than that in control group,P < 0. 001. 2.The correlation analysis of age,sex,education background and AD7c-NTP content( Pearson correlation analysis) showed that there was no significant correlation between urinary AD7c-NTP and the above factors;MMSE score and urinary AD7c-NTP were negative correlatively,the CDR score was positively correlated with AD7c-NTP. 3. The difference of urinary AD7c-NTP between mild and moderate AD group and severe AD group was statistically significant( F = 88. 756,P <0. 001),suggesting that the urinary AD7c-NTP increased with the severity of the disease. Conclusion The detection of AD7c-NTP in the urine of elderly AD patients can help for clinical diagnosis and assessment of the severity of AD.