淫羊藿苷对去卵巢骨质疏松大鼠骨细胞凋亡及骨组织OPG、RANKL mRNA表达影响

Effect of Icariin on Apoptosis of Bone Cells and Expression of OPG,RANKL and mRNA in Bone Tissue of Ovariectomized Rats with Osteoporosis

目的:探讨淫羊藿苷对去卵巢骨质疏松大鼠骨细胞凋亡及骨组织骨保护素(OPG)、核因子κB受体活化因子配体(RANKL)mRNA表达的影响。方法:选取健康雌性SD大鼠70只,采用去卵巢法建立骨质疏松模型,造模成功的51只大鼠随机分为模型组、尼尔雌醇组、淫羊藿苷组,各17只;对照组15只接受假手术,各组给予相应的药物治疗。对大鼠股骨骨密度(BMD)、骨矿含量(BMC)、血清生化指标水平、骨细胞及成骨细胞凋亡指数(AI)、骨组织OPG mRNA、RANKL mRNA、核因子κβ受体活化因子(RANK)mRNA表达水平进行检测。结果:治疗后,与模型组比较,各组大鼠股骨BMD、BMC水平较高,且淫羊藿苷组>尼尔雌醇组(P<0.05);各组大鼠血清骨钙素(BGP)、碱性磷酸酶(ALP)、钙(Ca)水平较高,且淫羊藿苷组>尼尔雌醇组(P<0.05);各组大鼠骨细胞AI、成骨细胞AI较低,且淫羊藿苷组<尼尔雌醇组(P<0.05);各组大鼠骨组织OPG mRNA表达水平较高,且淫羊藿苷组>尼尔雌醇组(P<0.05);各组骨组织RANKL mRNA、RANK mRNA表达水平较低,且淫羊藿苷组<尼尔雌醇组(P<0.05)。结论:淫羊藿苷能增加去卵巢骨质疏松大鼠骨密度和骨矿含量,改善血清BGP、ALP、Ca水平,抑制骨细胞及成骨细胞凋亡,可能与上调骨组织OPG mRNA表达,下调RANKL mRNA、RANK mRNA表达有关。

Objective:To analysis the effect of icariin on apoptosis of bone cells and expression of osteoprotegerin(OPG),nuclear factor kappa B receptor activator ligand(RANKL)mRNA in bone tissue of ovariectomized rats with osteoporosis. Methods:Seventy healthy female SD rats were selected,the osteoporosis model was established by ovariectomy,51 rats which successfully modeled were randomly divided into model group,nilestriol group and icariin group,17 rats for each group;15 rats in the control group underwent sham surgery,and all rats were given corresponding drug treatment. The femoral bone mineral density(BMD),bone mineral content(BMC),serum biochemical parameters,bone cell and osteoblast apoptosis index(AI),bone tissue OPG,mRNA,RANKL mRNA,nuclear factor kappa beta receptor activator(RANK)mRNA expression levels were detected. Results:After treatment,compared with model control group,the levels of BMD and BMC in the femur were higher in each group,and icariin group > nilestriol group(P<0.05);the serum osteocalcin(BGP),alkaline phosphatase(ALP),and calcium(Ca)levels were higher in rats of each group,and icariin group>nilestriol group(P<0.05);the bone cell AI and osteoblast AI were lower in each group,and icariin group<nilestriol group(P<0.05);the expression of RANKL mRNA,RANK mRNA in bone tissue of rats in each group was higher,and icariin group > nilestriol group(P<0.05);the expression of OPG mRNA in bone tissue of rats in each group were lower,and icariin group<nilestriol group(P<0.05). Conclusion:Icariin can increase bone mineral density and bone mineral content in ovariectomized rats with osteoporosis,improve the serum levels of BGP,ALP and Ca,inhibit the apoptosis of bone cells and osteoblasts,accelerate fracture healing,it may be associated with up regulation of OPG mRNA expression in bone tissue,and down regulation of RANKL mRNA and RANK mRNA expression.