平喘宁调节哮喘大鼠肺组织EGF、C-JUN干预气道重塑研究

Study on Pingchuanning in the Intervention of Airway Remodeling by Regulating EGF and C-JUN in Lung Tissue of Asthmatic Rats

目的:研究平喘宁对寒哮大鼠肺组织中ERK信号通路中EGF、C-JUN等相关蛋白以及C-JUN mRNA表达水平的影响,探讨平喘宁干预哮喘气道重塑的作用机制。方法:随机将雄性SD大鼠分为正常组、模型组、平喘宁小剂量组、平喘宁中剂量组、平喘宁大剂量组、桂龙咳喘宁组、地塞米松组,采用HE染色法检测实验后肺组织的病理学改变;采用SqRT-PCR法检测实验后肺组织中C-JUN mRNA的表达丰度;采用Wersten blot法检测实验后肺组织中EGF、C-JUN的相对表达量。结果:HE染色后,模型组较正常组有明显气道重塑现象;各治疗组气道重塑均较模型组得到适当缓解。通过Western blot法检测发现,与正常组相比,EGF蛋白的表达在平喘宁大剂量组无特异性变化(P>0.05),在其他各组均有增高(P<0.01);C-JUN蛋白的表达在除平喘宁中剂量组无特异性变化外(P>0.05),在其他各组均有增高(P<0.01);与模型组相比,EGF的表达在地塞米松组和平喘宁大剂量组、小剂量组中降低(P<0.01、P<0.05);C-JUN蛋白的表达在平喘宁大剂量组和平喘宁中剂量组中下降(P<0.01、P<0.05)。通过RT-PCR检测:与正常组相比,C-JUN mRNA的表达在各组中均增高(P<0.01);与模型组相比,C-JUN mRNA的表达在平喘宁大剂量组、平喘宁中剂量组中下降(P<0.01)。结论:平喘宁大剂量组与平喘宁中剂量组可降低寒哮大鼠肺组织中EGF、C-JUN蛋白和基因的表达,从而延缓气道重塑以治疗哮喘。

Objective:To study the effect of Pingchuanning on the expression of EGF,C-JUN and other related proteins and C-JUN mRNA in ERK signaling pathway in lung tissue of cold asthmatic rats,and to explore the mechanism of Pingchuanning on asthma airway remodeling. Methods:Male SD rats were randomly divided into model group,normal group,Pingchuanning high dose group,Pingchuanning middle dose group,Pingchuanning small dose group,Guilong Kechuanning group and dexamethasone group. The expression of C-JUN mRNA in lung tissue was measured by SqRT-PCR. The relative expression of EGF and C-JUN in lung tissue was detected by Wersten blot. The expression of C-JUN mRNA in lung tissue was detected by SqRT-PCR. Results:Compared with the normal group,the model group had obvious airway remodeling. Compared with the model group,the airway remodeling of the treatment groups were relieved. Compared with the normal group,the expression of EGF in the Pingchuanning had no specific change(P>0.05). The expression of EGF in the other groups was significantly higher than that in the normal group(P<0.01). The expression of C-JUN protein had no specific change in Pingchuanning middle dose group(P>0.05),increased in all other groups(P<0.01). Compared with model group, the expression of EGF decreased in dexamethasone group, Pingchuanning high dose group and low dose group(P<0.01,P<0.05);The expression of C-JUN protein decreased in Pingchuanning high dose group and Pingchuanning middle dose group(P<0.01,P<0.05). RT-PCR showed that the expression of C-JUN mRNA in all treatment groups were higher than that in the normal group(P<0.01). Compared with the model group, the expression of C-JUN mRNA decreased in the Pingchuanning high dose group and Pingchuanning middle dose group(P<0.01). Conclusion:Pingchuanning high and middle dose group can reduce the airway inflammation and delay airway remodeling in asthmatic rats by reducing the expression of EGF and C-JUN in lung tissue of cold asthmatic rats.