摘要
目的:建立舒冠通糖浆原药材盐肤木根中总鞣质的含量测定方法,并对产自福建的该药材进行鞣质含量的测定。方法:以《中华人民共和国药典》2015版四部中鞣质含量测定法(磷钼钨酸-干酪素法)为基础,在显色温度、显色时间、干酪素的用量及处理方法等方面进行改良,确定了含量测定方法,并用该法测定样品中总鞣质含量。结果:通过对鞣质含量测定各环节的考察,确定了原药材鞣质含量测定方法,并进行了方法学验证,没食子酸在1.00~12.00μg/mL与吸光度呈良好的线性关系,r=0.9985,操作重复性良好,RSD=2.89%,闽产盐肤木根中鞣质含量为22.1 mg·g-1(n=6,RSD=2.39%)。结论:经方法学验证,该方法可用于测定闽产盐肤木根中总鞣质的含量。
Objective:To establish the method for determination of total tannis of the root of Rhus chinensis from Fujian province and determine its content. Methods:An optimized method with which the content of total tannis was finally determined was developed based on the phosphomolybdium tungstic acid-casein reaction reported in Pharmacopoeia of the People’s Republic of China 2015,volumeⅣ. Incubation time and amount of the casein were modified. Results:The modified strategy achieved good results. The gallic acid had good linear relationship in the range of 1.00-12.00 μg/mL(r=0.9985). The repeatability was also satisfactory,with the RSD of 2.89%,total tannis of the root of Rhus chinensis from Fujian province was 22.1 mg·g-1(n=6,RSD=2.39%). Conclusion:According to the methodology validation,this method can be applied to the determination of the total tannis of the Rhus chinensis root from Fujian province.
作者
叶淼
吴献
林小栩
张举富
胡炜
许文
徐伟
YE Miao;WU Xian;LIN Xiaoxu;ZHANG Jufu;HU Wei;XU Wen;XU Wei(School of Pharmcy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China;Fujian Key Laboratory of Research and Development of Traditional Chinese Medicine Resources,Fuzhou 350122,Fujian,China)
出处
《辽宁中医药大学学报》
CAS
2019年第4期77-79,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(81803678)
福建省卫生计生中青年骨干人才培养项目(2017-ZQN-65)
福建省高校产学合作项目(2016Y4007)
福建中医药大学校管科研课题(Χ2016011-重点)
关键词
盐肤木
总鞣质
含量
改良的磷钼钨酸-干酪素法
Rhus chinensis
total tannis
content
modified phosphomolybdium tungstic acid-casein reaction