清肺透邪方对MPP小鼠炎症因子IL-10、IL-17疗效机制研究

Research of Therapeutic Mechanism of Qingfei Touxie Decoction on Inflammatory Factor IL-10 and IL-17 in MPP Mice

目的:通过肺炎支原体滴鼻方法建立感染幼龄Balb/c小鼠MPP模型,采用Elisa法检测小鼠外周血中IL-17、IL-10表达水平变化,观察清肺透邪方对肺炎支原体肺炎(MPP)小鼠治疗效果及其时效关系,为临床治疗肺炎支原体肺炎提供实验依据。方法:90只幼龄Balb/c小鼠随机分为正常组、模型组、阿奇霉素组、中药组和中药+阿奇组,共5组,每组18只,除正常组外,其余4组均建立MPP模型。连续滴鼻3 d后,阿奇霉素组小鼠予阿奇霉素0.2 mL/(d·20 g)灌胃,中药组予0.2 mL/(d·20 g)灌胃,中药+阿奇组予分别阿奇霉素和清肺透邪方颗粒剂各0.2 mL/(d·20 g)灌胃,正常组与模型组予等体积生理盐水灌胃。分别于给药后第3、7、14天处死取材,每组6只采用Elisa法检测外周血中IL-10、IL-17表达水平变化,观察清肺透邪方对MPP小鼠的治疗作用及其时效关系。结果:感染第3、7天时,5组间IL-10水平比较均无统计学差异;感染第14天时模型组IL-10水平均低于正常组(P<0.05),3组给药组均明显高于模型组(P<0.05),且中药+阿奇组明显高于其他两组给药组(P<0.05)。感染第3天时,模型组、阿奇霉素组、中药组、中药+阿奇组小鼠IL-17细胞水平含量均高于正常组(P<0.05),3组给药组均低于模型组(P<0.05),且给药组组间比较无统计学差异;感染第7、14天时模型组与3组给药组小鼠IL-17水平均高于正常组(P<0.05),且中药+阿奇组明显低于其他两组给药组(P<0.05)。结论:清肺透邪方可以有效调控MPP小鼠IL-10、IL-17因子的表达,且联用阿奇霉素效果更优,这可能是清肺透邪方防治MPP以及减轻其炎症反应的作用机制之一。

Objective:To establish a model of MPP in Balb/c mice infected with mycoplasma pneumoniae by nose dropping.The expression of IL-10 and IL-17 in peripheral blood of mice was detected by Elisa method.The therapeutic effect of Qingfei Touxie Decoction on mycoplasma pneumoniae pneumonia in mice and its time-effect relationship were observed,so as to provide experimental basis for clinical treatment of mycoplasma pneumoniae pneumonia.Methods:Ninety young Balb/c mice were randomly divided into five groups:normal group,model group,azithromycin group,traditional Chinese medicine group and traditional Chinese medicine+azithromycin group.Eighteen mice in each group were divided into five groups.MPP models were established in all four groups except the normal group.After three consecutive days of nasal drip,mice in azithromycin group were given 0.2 mL/(d·20 g)intragastric administration of azithromycin,0.2 mL/(d·20 g)intragastric administration of traditional Chinese medicine group,0.2 mL/(d·20 g)intragastric administration of azithromycin and Qingfei Tuixie Decoction granules respectively,and 0.2 mL/(d·20 g)intragastric administration of normal saline and model group.Samples were sacrificed on the 3 rd,7 th and 14 th day after administration.The expression levels of IL-17 and IL-10 in peripheral blood were detected by Elisa method.The therapeutic effect of Qingfei Touxie Decoction on MPP mice and its time-effect relationship were observed.Results:At the 3 rd and 7 th day of infection,there was no significant difference among the five groups in the levels of IL-10.On the 14 th day of infection,the IL-10 level in the model group was lower than that in the normal group(P<0.05),and the IL-10 level in the three drug groups of drug administration was significantly higher than that in the model group(P<0.05),and that in the Chinese medicine+azithromycin group was significantly higher than that in the other two groups of drug administration(P<0.05).On the 3 rd day of infection,the levels of IL-17 cells in model group,azithromycin group,traditional Chinese medicine group and traditional Chinese medicine+azithromycin group were higher than those in normal group(P<0.05),and the levels of IL-17 in three groups of drug administration were lower than those in model group(P<0.05).There was no statistical difference between the three groups.On the 7 th and 14 th day of infection,the levels of IL-17 in model group and three groups of drug administration were higher than those in normal group(P<0.05),and the traditional Chinese medicine+azithromycin group was significantly lower than the other two groups of drug administration(P<0.05).Conclusion:Qingfei Touxie Decoction can effectively regulate the expression of IL-10 and IL-17 in MPP mice,and the combination of azithromycin is more effective,which may be one of the mechanisms of Qingfei Touxie Decoction in preventing MPP and reducing its inflammatory reaction.