固定化酵母菌对活性艳蓝KN-R的脱色研究

Study on Decolorization of Reactive Brilliant Blue KN-R by Immobilized Cells of Yeast

采用海藻酸钙加活性碳的方法对酵母菌进行固定化。正交试验结果表明 ,三者合适配比为海藻酸钠 3 % ,活性碳 10 % ,氯化钙 4%。此方法制得的凝胶颗粒机械强度好、经久耐用。固定化酵母菌凝胶小球最适条件为 :培养时间为 48h ,培养温度为 3 7℃ ,培养基pH为 3 0 ,凝胶量为 10 g。固定化细胞经多次脱色后能重复使用 ,4次脱色后其脱色率还达 79 2 %。

Yeast was immobilized in sodium alginate and activated carbon The results of orthogonal tests showed that optimal concentrations of the three substances were 3% sodium alginate, 10% activated carbon and 4% CaCl 2,respectively The gel beads were durable and had great mechanical strength The suitable conditions for immobilized cells were determined as follows: culture time 48h, temperature 37℃ and pH 3 0, respectively The inoculum amount was 10g The immobilized cells could be reused after the forth decolorization and the decolorizing rate is still 79 2%