摘要
A newly isolated strain EL31410, producing elastase (E.C3.4.4.7) with h igh elastolytic activity was identified as Bacillus sp. In the medium opt imizat ion, it was found that wheat bran and soybean flour hydrosate were the best crud e carbon and nitrogen source for enzyme production, respectively. Addition of co rn steep flour can affect the bacterium growth and elastase production. A fracti onal factorial design was applied to study the main factors that affect the enzy me production, and central composite experimental design and response surface me thodology were adopted to derive a statistical model for the effect of wheat bra n and soybean flour hydrosate on elastase production. The experimental results s howed that wheat bran had positive effect but soybean flour hydrosate had neg ative effect, on enzyme production. An initial concentration of 3.4%(w/v) wh eat b ran and 9.4%(v/v) soybean flour hydrosate were found to be optimal for enzyme pr oduction in batch culture. The time course of elastase production in the optimiz ed medium composition was also described.
A newly isolated strain EL31410,producing elastase(E.C3.4.4.7) with high elastolytic activity was identified as Bacillus sp.In the medium optimization,it was found that wheat bran and soybean flour hydrosate were the best crude carbon ad nitrogen source for enzyme production,respectively.Addition of com steep flour can affect the bacterium growth and elastase production.A fractional factorial design was ap-plied to study the main factors that affect the enzyme production,and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production.The experimental results showed that wheat bran had positive cffect but soybean flour hydrosate had negative effect,on enzyme production.An initial concentration of 3.4%(w/v) wheat bran and 9.4%（v/v) soybean flour hydrosate were found to be optimal for enzyme produc-tion in batch culture.The time course of elastase production in the optimized medium composition was also de-scribed.
